14TSB_SynBio Automated Gene Assembly From Codons to Complete Genes and Pathways

从更小的DNA片段组装基因或产生基因突变组合文库的能力是合成生物学领域的基础。它们的制造存在不同的方法，但与其他方法相比，酶组装产生始终如一的高质量产品，这对于改善特性（无论是改善菌株表型还是改善蛋白质功能/产量）的下游工艺至关重要。以高通量、自动化的方式实现这一目标的潜力将是合成生物学领域商业化的强大而有价值的工具。在这方面，我们之前已经开发了一种技术，可以通过酶加密码子三胞胎来创建基因片段的随机延伸。我们现在希望进一步研究以自动化方式将多样化的基因片段，特别是抗体可变环组装成完整基因的程序。帝国理工学院正在自动化组装方法，通过连接密码子到基因和途径的规模，从而有可能开辟新的市场领域，从而使项目成为可能。

The ability to assemble genes from smaller DNA fragments or generate combinatorial libraries of genetic mutations is fundamental to the field of synthetic biology. Different methodologies exist for their fabrication but enzymatic assembly produces consistently high quality products compared to other methods which is essential for downstream processes for improved characteristics (whether this is for improved strain phenotype or an improved protein function/yield). The potential to achieve this in a high throughput, automatable fashion would be a powerful and valuable tool for commercialisation in the synthetic biology arena. In this respect, we have previously developed a technology that can create random stretches of gene fragments using enzymatic additions of codon triplets. We now wish to investigate further procedures to assemble diversified gene fragments, in particular antibody variable loops, into whole genes in an automated fashion. Imperial College is automating assembly methods that would be enabling for the project by bridging the scale from codons to genes and pathways, thereby potentially the opening up new market areas.