BONE MARROW TRANSPLANTATION FOR HIV PATIENTS WITH CANCER

该项目要求支持临床前和临床研究 评估骨髓移植的作用， 抗病毒化疗治疗HIV-1阳性患者 血液恶性肿瘤 有三个具体目标。 在目标1中，我们计划确定强化治疗是否遵循 通过BMT可以消除HIV-1感染细胞的储存库， 3 '-叠氮基-3'-脱氧胸苷（AZT）可预防 从同种异体骨髓中获得的移植供体细胞。 在 目的2，我们计划确定哪些淋巴造血细胞， HIV-1阳性患者携带病毒，以确定是否 病毒存在于干细胞中， 感染HIV-1的患者在不同的阶段， 疾病 在目标3中，我们计划研究多能性 不含HIV-I的淋巴造血干细胞可以通过体外培养产生。 体内骨髓操作和“净化”技术。 如果这 目标是成功的，这将导致考虑自体BMT 作为HIV-1感染患者的治疗方法。

This project requests support for preclinical and clinical studies evaluating the role of bone marrow transplantation (BMT) and antiviral chemotherapy in the treatment of HIV-l positive patients with hematologic malignancy. Three specific aims are addressed. In Aim 1, we plan to determine whether intensive therapy followed by BMT can eliminate the reservoir of HIV-l infected cells and if 3'-azido-3'deoxythymidine (AZT) can prevent infection of transplanted donor cells obtained from allogeneic bone marrow. In Aim 2, we plan to determine which lympho-hematopoietic cells in HIV-l positive patients carry the virus to determine whether the virus is present in stem cells and what fraction of cells are infected with HIV-l in patients at different stages of their disease. In Aim 3, we plan to investigate whether pluripotent lympho-hematopoietic stems free of HIV-l can be generated with ex vivo bone marrow manipulation and "purging" techniques. If this aim is successful it will lead to consideration of autologous BMT as therapy for patients with HIV-l infection.