The diagnostic window for detection of viruses infecting salmon in erythrocytes.

对感染鱼类的病毒的诊断通常是通过检测鱼组织中病毒的核酸来完成的。这种方法忽略了越来越多的证据表明这些病毒可以在血液中复制。如果血液可以作为诊断样本，它将简化并可能加快诊断测试。我们想探讨五种感染鲑鱼的病毒（ISAV， SAV， PRV，PMCV， IPMV）在鲑鱼红细胞中的复制。我们想要评估这些病毒中哪些在红细胞中表现出良好的复制，以及是否可以得出在活鲑鱼中检测它们的诊断窗口的结论。我们还想探索病毒感染红细胞是否会引起免疫反应，这将表明首次接触感染因子的红细胞也参与了抵抗感染的第一线防御。我们想监测的另一个特征是感染红细胞引起的形态学改变。如果这些病毒在红细胞中的复制可以被证实，它将导致简化病毒诊断的方式。为了进一步标准化测试，我们希望开发用于鱼类诊断实验室分子诊断外部质量评估的材料。这是绝对必要的，因为认可服务不能评估单个实验室的表现，也不能将单个实验室的表现与其他实验室进行比较。总之，在人类感染病毒的诊断中已经确立的质量评估的整个概念，在鱼类病毒的实验室现场检测中是缺失的。为此，我们已将格拉斯哥的QMCD纳入我们的联盟，该联盟在开发EQA面板方面拥有长期的专业知识。总的来说，这个项目的结果将有助于简化和优化对感染鲑鱼的病毒的分子检测。

Diagnosis of viruses infecting fish is often done by detecting the nucleic acids of the viruses in fish tissues. This approach ignores increasing evidence that these viruses can replicate in blood. If blood could be used as a diagnostic sample it would simplify and possibly also speed up diagnostic testing.We want to explore the replication of five viruses infecting salmon (ISAV, SAV, PRV,PMCV, IPMV) in salmon erythrocytes. We want to assess which of these viruses show good replication in erythrocytes and if conclusions for a diagnostic window for their detection in live salmon can be drawn. We also want to explore if virus infection of the erythrocytes illicits an immune response, which would show that erythrocytes which are in first contact with the infectious agents are also involved in front line defense against infection. Another feature we would like to monitor are the morphological changes induced in infected erythrocytes.If replication of these viruses in erythrocytes can be confirmed it would lead the way to simplifying virus diagnostics. To additionally standardize testing we want to develop material for external quality assessments of molecular diagnostics in fish diagnostic laboratories. This is absolutely necessary since accreditation services can not assess how individual laboratories are performing nor are individual laboratories able to compare their performance to others. In short the whole concept of quality assessment, which is well established in the diagnostics of viruses infecting humans, is missing in the laboratory scene testing for fish viruses. To this end we have included QMCD from Glasgow into our consortium which has a long standing expertise in developing EQA panels.Overall the outcomes of this project will help to simplify and to optimize molecular detection for viruses infecting salmon.