ANALYSIS AND PHYSIOLOGY OF ISOFLAVONES IN SOYBEANS

流行病学数据表明，饮食是影响健康的重要因素 解释东方乳腺癌发病率下降5-8倍的原因 国家与西方世界的比较，以及最近的大豆蛋白 研究发现，摄入量与乳腺癌风险降低有关。 绝经前的东方女性。我们压倒一切的假设是“大豆 蛋白质有益于预防和/或治疗乳房 癌症，因为存在非类固醇雌激素的 已经显示出抗癌作用的异黄酮类。 大豆蛋白对实验动物肿瘤生长的抑制作用 乳腺癌模型，以一种剂量依赖的方式，并且效果 与大豆异黄酮类摄入量相关。这项提议旨在澄清 我们对大豆的吸收、代谢和生理的认识 人体内的异黄酮类。确定肠道新陈代谢程度 以及主要大豆的吸收、未结合形式和结合形式 大豆异黄酮类、大豆苷元和染料木素将用于健康人群 将收集绝经前妇女以及尿液、血清、胆汁和粪便。 按质量分析异黄酮组分的定时间隔 光谱分析。通过现场特定的交付，在高和 低碳水化合物日粮不同代谢水平和生物利用度 将对大豆异黄酮类化合物的形式进行比较。我们已经证明了慢性 大豆摄取影响月经周期的激素调节 女人。为了确定这些影响是否归因于异黄酮类化合物， 与剂量相关，并与循环浓度和 异黄酮类、异黄酮类大豆蛋白的尿液排泄 将给健康的绝经前妇女服用成分，尿液， 收集血清和粪便。确定是否与 内源性雌激素、异黄酮类的局部摄取和分泌 在人的乳房中，异黄酮会在乳房中测量浓度 绝经前非哺乳期妇女的囊液和乳头抽吸物 大豆异黄酮类摄入前后。异黄酮类成分 和不同地区原产大豆品种的组成 世界将被确定，它们对温度的稳定性将用于 将对烹饪进行考试。响应对例行公事的迫切需要 我们将开发生物样品中异黄酮类的测定方法 一种使用成功用于类固醇的策略的特异性免疫分析 荷尔蒙检测。我们还将改进现有的质谱学方法。 以允许测定组织中的异黄酮类。结果是 这些研究将使未来饮食的最适当设计成为可能。 使用大豆蛋白的干预研究。

Epidemiological data indicate that diet is an important factor in explaining the 5-8 fold lower incidence of breast cancer in Oriental countries compared with the Western world, and recently soy protein intake was found to be associated with a decreased risk for breast cancer in premenopausal Oriental women. Our overriding hypothesis is that "Soy protein is beneficial in the prevention and/or treatment of breast cancer, because of the presence of non-steroidal estrogens of the isoflavone class that have demonstrated anticancer actions." We have shown that soy protein will inhibit tumor growth in experimental animal models of breast cancer, in a dose-dependent fashion, and that the effect is correlated with soy isoflavone intake. This proposal seeks to clarify our understanding of the absorption, metabolism and physiology of soy isoflavones in humans. To establish the degree of intestinal metabolism and absorption, unconjugated and conjugated forms of the principal soy isoflavones, daidzein and genistein, will be administered to healthy premenopausal women, and urine, serum, bile, and feces, will be collected at timed intervals for analysis of isoflavone concentrations by mass spectrometry. By site-specific delivery, under conditions of high and low carbohydrate diets, the metabolism and bioavailability of different forms of soy isoflavones will be compared. We have shown that chronic soya ingestion affects the hormonal regulation of the menstrual cycle of women. To determine whether these effects are due to isoflavones, are dose-dependent and are related to the circulating concentrations and urinary excretion of isoflavones, soy proteins differing in isoflavone composition will be given to healthy premenopausal women, and urine, serum and feces collected. To determine whether in common with endogenous estrogens, local uptake and secretion of isoflavones occur in the human breast, concentrations of isoflavone will be measured in breast cyst fluid and nipple aspirates of non-lactating premenopausal women before and following soy isoflavone ingestion. The isoflavone content and composition of soybean varieties indigenous to different regions of the world will be determined and their stability to temperatures used in cooking will be examined. In response to a critical need for a routine method for measuring isoflavones in biological samples we will develop a specific immunoassay using strategies successfully employed for steroid hormone assays. We will also refine existing mass spectrometric methods to permit the determination of isoflavones in tissues. The results of these studies will permit the most appropriate design of future dietary intervention studies using soy protein.