TOXIC EFFECT OF CHLORINATED AND PYRETHROID PESTICIDES

在本研究项目中，我们建议研究氯化物的毒性效应， 和拟除虫菊酯类农药主要是钙调节过程 神经系统，肝脏和心脏 主要实验动物 使用的是大鼠，但小鼠，豚鼠，仓鼠和一些 还将使用无脊椎动物物种。 主要研究的化合物有 滴滴涕、毒杀芬、氯氰菊酯、狄氏剂、艾氏剂、萘甲草胺、环氧化萘甲草胺、 丙烯菊酯、氯菊酯、氰戊菊酯、氯氰菊酯和溴氰菊酯。 在 肝脏的情况下，主要重点将放在长期 这些化学物质对肝细胞钙转运功能的影响。 同时也研究了它们对间隙结的影响 已知其受钙浓度控制， 功能协调发展的 这些农药对细胞间的影响程度 识别和通信将被确定。 的情况 神经系统，我们计划研究它们对各种钙的影响， 调节系统，如钠钙交换，特别是部分 与ATP利用率有关，对Ca-Mg ATP酶的影响。 在后一种情况下 与钙调素相互作用的变化以及 将检查释放和神经分泌。 至于环二性型 氯化农药，我们计划研究它们对GABA-氯化物的影响， 渠道系统。 将特别强调对下列问题的研究： 苦毒素受体 该项目还将涵盖以下领域： 农药对心脏生化的影响，特别是与其 影响钙调节。

In this research project we propose to study toxic effects of chlorinated and pyrethroid pesticides mainly on calcium regulating processes in the nervous system, the liver and the heart. The main experimental animals to be used is the rat, but the mouse, the guinea pig, the hamster and some inverterbrate species will also be used. The compounds mainly studied are DDT, toxaphene, chlordane, dieldrin aldrin, neptachlor, neptachlor epoxide, allethrin, permethrin, fenvalerate cypermetnrin and decamethrin. In the case of the liver, the major emphasis will be placed on the long term effects of these chemicals on calcium transport functions of hepatocytes. Also an effort will be made to study their effects on the gap-junction which is known to be controlled by calcium concentration for its functions. The extents of the influence of these pesticides on cell-cell recognition and communication will be determined. In the case of the nervous system, we plan to study their influence on various calcium regulating systems such as Na-Ca exchange, particularly the portion relating to ATP utilization, and on Ca-Mg ATPase. In the latter case changes in its interaction with calmodulin as well as in transmitter release, and neurosecretion will be examined. As for cyclodience-type chlorinated presticides we plan to examine their effects on GABA-chloride channel systems. A particular emphasis will be placed on the studies on the picrotoxinin receptor. This project will also cover the area of pesticide effects on cardiac biochemistry especially as related to their influence on calcium regulation.