CALCIUM BINDING PROTEINS AND CALCIUM HOMEOSTASIS

维生素D诱导的糖尿病的确切和普遍接受的作用 钙结合蛋白（CaBP）目前还没有，尽管 事实上，自发现以来已经过去了10多年。 的结果 近年来的研究表明， 维生素D依赖的钙代谢，但是，了解确切的 这一作用的机制在很大程度上受到关于 CaBP的免疫细胞学定位。 拟议的调查是 旨在系统地调查协议中的变量， 在以前的本地化研究中使用，目的是确定 导致不同结果的因素。 有了这些信息，A 将设计可靠的细胞学定位方案。 国产化 在不同营养条件下， 已知改变肠钙转运和 CaBP含量。 CaBP将在另外两个国家使用相同的协议进行定位。 钙运输器官，肾脏和壳腺的蛋鸡。 的 蛋白质也会定位在大脑中。 无论是光显微镜还是 将研究CaBP的超微结构定位。 可靠信息 关于这些组织中的CaBP位点将极大地有助于确定 蛋白质在钙代谢中的功能，这反过来可能是有价值的 在获得更好的理解改变钙代谢的原因， 不同的疾病状态。 本研究还提出了一种分离纯化线粒体钙结合蛋白的方法， 蛋白质，糖蛋白（CaBGP），然后产生单特异性抗血清， 蛋白 CaBGP的细胞学定位将作为以下功能进行研究： 钙化软骨中的矿化状态。 可能参与 钙骨钙素在钙化反应将进行研究。

A definitive and generally accepted role for the vitamin D-induced calcium-binding protein (CaBP) is not available at the present time despite the fact that more than 10 years have elapsed since its discovery. The results of research in the intervening years have suggested an involvement of the protein in vitamin D-dependent calcium metabolism, but, an understanding of the exact mechanism of that role suffers to a large degree from the controversy concerning the immunocytological localization of CaBP. The proposed investigations are designed to systematically investigate the variables in the protocols which have been used in previous localization studies with the goal of identifying the factor(s) responsible for the divergent results. With that information, a reliable cytological localization protocol will be designed. The localization of intestinal CaBP will be investigated under different nutritional and physiological conditions known to alter both intestinal calcium transport and CaBP content. CaBP will be localized using the same protocol in two other calcium-transporting organs, the kidney and shell gland of the laying hen. The protein will also be localized in brain. Both the light microscopic and the ultrastructural localization of CaBP will be investigated. Reliable information concerning the CaBP site(s) in these tissues will greatly aid in defining the function(s) of the protein in calcium metabolism which in turn may be of value in gaining a better unerstanding of the causes of altered calcium metabolism in different disease states. A study is also proposed to isolate and purify a mitochondrial calcium binding protein, a glycoprotein (CaBGP), then raise a monospecific antiserum against the protein. The cytological localization of CaBGP will be studied as a function of the mineralization state in calcifying cartilage. The potential involvement of CaBGP in calcification reactions will be investigated.