AGE-RELATED RESPONSE OF SALIVARY GLANDS TO ISOPROTERENOL

有人建议利用唾液腺作为体内模型。 β-肾上腺素能神经递质调节机制的研究(S) 受体介导的两种不同基因在同一细胞中的表达 细胞，在正常发育期间表达的细胞(LM)和 另一种是原癌基因(c-fos)，即肿瘤的发育模式。 这是个未知数。对这些因素的更多了解 调节一个基因的表达，该基因在 唾液腺的正常发育和原癌基因 它们可以被相同的β-肾上腺素能诱导表达 激动剂，以及它们表达改变的方式 对于理性地理解两者都是必不可少的 正常和异常的细胞机制。 我们最近克隆了LM分泌物的编码基因 蛋白质，并建议使用该克隆来检测以下内容 具体目标： 一、确定该基因表达的发育模式 Lm基因，并将其表达与卵巢癌指标进行相关性分析。 唾液腺在体内的分化和生长(两者 生理和刺激)，以及 II.测定Lm基因(S)的数量，分离和鉴定 鉴定Lm基因(S)，以鉴定Lm基因，以及 对LM基因进行染色体定位(S)，以及 研究β-肾上腺素能的机制(S) 激动剂早熟诱导基因(Lm)表达 在正常发育期间，并对这些进行比较和对比 原癌基因(c-fos)的作用机制 已知在正常发育过程中表达，但可以 由相同的因素诱导表达的。 唾液腺出生后发育的研究概况 将是未来实验的基础，旨在研究这些 衰老和衰老动物的参数。

It is proposed to utilize salivary glands as an in vivo model for investigating the mechanism(s) regulating the beta-adrenergic- receptor mediated expression of two different genes in the same cell, one which is expressed during normal development (LM) and the other a proto-oncogene (c-fos), the developmental pattern of which is unknown. An increased understanding of the factors regulating the expression of a gene which is expressed during normal development of salivary glands, and a proto-oncogene both of which can be induced to express by the same beta-adrenergic agonist, and the manner in which their expression may be altered is essential for rational approaches toward understanding both normal and aberrant cellular mechanisms. We have recently cloned the cDNA coding for the LM secretory protein and propose to use the clone to examine the following specific aims: I. to determine the developmental pattern of expression of the LM gene and to correlate this expression with indices of differentiation and growth of salivary glands in vivo (both physiologic and stimulated), and II. to determine the number of LM gene(s), to isolate and characterize the LM gene(s), to characterize the LM mRNA, and chromosomally localize the LM gene(s), and III. to examine the mechanism(s) by which a beta-adrenergic agonist precociously induces a gene (LM) which is expressed during normal development, and compare and contrast these mechanisms with those of a proto-oncogene (c-fos) which is not known to be expressed during normal development, but can be induced to express by the same agent. The studies outlined on postnatal development of salivary glands will be the basis of future experiments designed to examine these parameters in aging and senescent animals.