GLANDULAR KALLIKREIN IN HUMAN SALIVA

腺激肽释放酶是一种“胰蛋白酶样”调节性丝氨酸蛋白酶 它存在于各种组织和生物体液中 包括尿液血液和唾液 虽然具体 这种酶的生理功能还没有被严格地 已建立，它已被建议参与血压 调节，控制钠转运，调节顺利 不依赖于激肽形成的肌肉收缩， 肽激素的加工。 激肽释放酶活性发现， 人唾液是腺激肽释放酶类型， 是下颌下腺和腮腺分泌物的产物 虽然在口腔病理生理学中的作用尚未确定， 几项研究表明，这种酶的分泌进入 唾液可受到全身生理学的影响，因此， 唾液中腺激肽释放酶浓度的变化 可能是特定疾病过程的重要监测器。 在 此外，系统性腺激肽释放酶的来源还没有被证实。 但有证据表明，在动物模型系统中 这表明唾液腺可能是 在循环中发现的腺激肽释放酶。 研究 在本申请中提出的技术方案集中于以下方面 问题：（1）哪些因素影响腺激肽释放酶水平 在从正常人群中获得的混合唾液中;（2） 混合唾液中腺激肽释放酶的浓度提供了一种 转移性疾病的有意义的指数，以及 腺激肽释放酶水平升高的肿瘤治疗 从患有实体瘤的受试者获得的混合唾液 唾液腺是否是口腔内分泌的主要来源 在血液中发现的腺激肽释放酶。 获得的这一信息 从这些研究中不仅可以深入了解 腺激肽释放酶可用于全身作用，但也将 提供使用变更所需的背景信息， 唾液腺激肽释放酶的水平，以确定和监测 系统病理生理学的特定变化。

Glandular kallikrein is a 'tryptic-like' regulatory serine protease whic is found in a wide variety of tissue and biological fluids including urine, blood and saliva. Although a specific physiological function for this enzyme has not been rigorously established, it has been suggested to be involved in blood pressure regulation, the control of sodium transport, regulation of smooth muscle contraction independent of kinin formation and the processing of peptide hormones. The kallikrein activity found in human saliva is of the glandular kallikrein type and appears to be a product of both submandibular and parotid gland secretion. Although a role in oral pathophysiology has not been identified, several studies suggest that the secretion of this enzyme into saliva can be influenced by systemic physiology and, hence, changes in the concentration of glandular kallikrein in saliva might be an important monitor of specific disease processes. In addition, the source of systemic glandular kallikrein has not been identified but there is evidence obtained in animal model systems which suggest that the salivary glands might be responsible for the glandular kallikrein found in the circulation. The research proposed in this application is focused toward the following questions: (1) What factors influence glandular kallikrein levels in mixed saliva obtained from a normal population; (2) Can the concentration of glandular kallikrein in mixed saliva provide an meaningful index of metastatic disease and what is the effect of oncology therapy on the elevated levels of glandular kallikrein in mixed saliva obtained from subjects with solid tumors distant from the oral cavity; and (3) Are salivary glands a major source of the glandular kallikrein found in blood. This information obtained from these studies will not only provide insight into the source of glandular kallikrein available for systemic action but also will provide background information necessary for using changes in salivary levels of glandular kallikrein to identify and monitor specific changes in systemic pathophysiology.