ULTRASTRUCTURAL HISTOPATHOLOGY OF HUMAN DENTAL ENAMEL

目前的研究涉及利用关于 牙釉质的超微结构形态和性能 前几年的这个项目，以获得了解搪瓷 畸形、釉质晶体溶解和釉质修复。 我们的长期 假设是釉质晶体的成核，生长，取向， 和排列（进入棱镜）取决于 晶体到有机基质和成釉细胞。 我们的目标是澄清 这些釉质组织超微结构排列和紊乱 发育和成熟过程中正常和病理状态的成分 搪瓷。 TEM结果将与其他观测结果直接相关 和技术[如临床、接触显微放射照相术（CMR）、光 显微镜（LM）和扫描电子显微镜（SEM）]。 我们的具体 目的是（a）使用在发育中的大鼠磨牙中诱导的成釉细胞下囊肿 作为检查超微结构釉质组织病理学的有用模型，和（B） 确定最佳的治疗方法，通过澄清釉质修复， 釉质组织的超微结构，特别是在 脱矿/矿化。 大鼠磨牙发育中的造釉细胞下囊肿- NaF -使用钠的研究 氟化物诱发囊肿和有缺陷的牙釉质将扩大到以下 回收过程，评估表面，确定密度和晶体尺寸， 分泌期和成熟期。 二膦酸盐-研究集中在 分泌和成熟过程中的细胞效应与直接晶体效应 阶段 O2水平-新生大鼠将处于低氧环境中， 确定单独和组合对大鼠釉质发育的影响 与氟化钠和二膦酸盐。 成熟人牙釉质-脱矿、再矿化、缺陷： 二膦酸盐-表面效应-使用2种二膦酸盐的实验 (EHDP，Cl 2 MDP）在成熟釉质表面上，在酸蚀刻之前和之后。 SEM结果将补充CMS和TEM。 脱矿/再矿化/龋齿-牛的初始实验 釉质将被重新定向到成熟人釉质的整体处理。 将通过CMR和TEM评估变化，并与不稳定和 正常的牙釉质 釉质簇-酸性（TCA）和EDTA（pH 7.2） 将修改实验以确定剩余的 晶体 组织学-乳牙和恒牙釉质 将检查缺损并与对照组和大鼠磨牙进行比较 实验

The research is currently concerned with utilizing the information on the ultrastructural morphology and properties of tooth enamel developed in previous years of this project to gain an understanding of enamel malformation, enamel crystal dissolution, and enamel repair. Our long-term hypothesis is that the enamel crystal's nucleation, growth, orientation, and arrangement (into prisms) is dependent on a unique relationship of the crystal to the organic matrix and the ameloblast. It is our aim to clarify the ultrastructural arrangement and disarrangement of these enamel tissue components in normal and pathological states in developing and mature enamel. TEM results will be directly correlated with other observations and techniques [e.g. clinical, contact microradiography (CMR), light microscopy (LM), and scanning electron microscopy (SEM)]. Our specific aims are (a) using the subameloblastic cyst induced in developing rat molar as a useful model to examine ultrastructural enamel histopathology, and (b) determining optimal treatments for enamel repair by clarifying the ultrastructure of enamel tissue especially during demineralization/remineralization. Subameloblastic Cysts in Developing Rat Molar - NaF - studies using sodium fluoride to induce cysts and defective enamel will be extended to follow recovery process, evaluate surface, determine density and crystal size at secretory and maturation stages. Diphosphonates - studies focused on cellular versus direct crystal effects during secretory and maturation stages. O2 levels - newborn rats will be in a reduced oxygen atmosphere to determine effects of rat enamel development both alone and in combination with NaF and diphosphonates. Mature Human Enamel - Demineralized, Remineralized, Defective: Diphosphonates - Surface Effects - experiments using 2 diphosphonates (EHDP, Cl2MDP) on mature enamel surfaces, before and after acid etching. SEM results will be supplemented with CMS and TEM. Demineralization/Remineralization/Caries - initial experiments with bovine enamel will be redirected to en bloc treatment of mature human enamel. Changes will be evaluated by CMR and TEM and compared with precarious and normal enamel. Enamel Tufts - ongoing acid (TCA) and EDTA (pH 7.2) experiments will be modified to determine distribution of remaining crystals. Histopathology - deciduous teeth and permanent teeth with enamel defects will be examined and compared to controls and to rat molar experiments.