HOST-BACTERIAL INTERACTIONS IN PERIODONTAL DISEASE

人类牙周炎是一种慢性牙周炎性疾病。 以疾病活动的爆发和更长的时期为特征 减刑。结扎诱导的牙周炎提供了一个类似的模型 可以加速发病的微生物区系。上一首 研究表明，热致死类杆菌免疫 猕猴对结扎性牙周炎的保护作用 通过减少猕猴的重新定殖率和减少 牙槽骨丢失与假免疫、结扎和免疫相比， 单组对照。这项建议扩展了这些先前的观察结果，并 试图增强“保护性”的体液反应，并评估 细菌因素是改变致病机制的主要因素。 结扎术引起的牙周炎。具体目标是：1.隔离 猕猴(B.macacae)囊膜部分。2.确定 分数(S)最有可能引起保护性反应。猴血清 可从先前资助的研究和兔抗血清中获得 这些组分将通过体外试验进行制备和评价 对殖民和传染性的潜在影响。这些措施包括 各组分的抗血清对调离、共聚集的影响 与其他口腔细菌，以及与上皮细胞的黏附。3.审查 所选细菌组分免疫对小鼠免疫的影响 猴牙周结扎性牙周炎。这将被描述为 临床、组织学、微生物学和免疫学基础。 4.评估免疫如何在结扎期间提供保护。因素 要考虑的因素包括细菌、血清对组织的侵袭 细菌的调理作用、细菌共聚集和细菌 黏附于上皮细胞。 这一建议将使我们更好地理解 牙周炎，并有潜在的治疗价值。

Human periodontitis is a chronic inflammatory disease of the periodontium characterized by bursts of disease activity and longer periods of remission. Ligature-induced periodontitis provides a model with a similar microflora in which the rate of disease can be accelerated. Previous studies have demonstrated that immunization with heat killed Bacteroides macacae provides some protection in the ligature induced periodontitis model by reducing both the recolonization by B. macacae and the extent of alveolar bone loss compared to the sham-immunized, ligated and immunized, mon-ligated controls. This proposal extends these earlier observations and attempts to enhance the "protective" humoral response and evaluate which bacterial factors are responsible for modifying the pathogenesis of ligature induced periodontitis. The specific aims are to: 1. Isolate capsular and membrane fractions from B. macacae. 2. Identify the fraction(s) most likely to evoke a protective response. Monkey sera available from the previous grant supported studies and rabbit antisera to the fractions will be prepared ad evaluated by in vitro assays for their potential influence on colonization and infectivity. These will include the effects of antisera to the fractions on opsonization, coaggregation with other oral bacteria, and adherence to epithelial cells. 3. Examine the effects of immunization with the selected bacterial fraction on ligature-induced periodontitis in monkeys. This will be characterized on the basis of clinical, histological, microbiological and immunological. 4. Evaluate how immunization confers protection during ligation. Factors to be considered include tissue invasion by the bacteria, serum opsonization of the bacteria, bacterial co-aggregation, and bacterial adherence to epithelium. This proposal would provide a better understanding of the pathogenesis of periodontitis and would potentially be of value in treatment.