BACTERIAL PROTEINASES IN PERIODONTAL DISEASE

成人牙周病的发展被认为是 与生物体局部分泌的蛋白酶有关 秦氏卟啉单胞菌进入牙龈腔。这些酶 可以降解一系列重要的蛋白质，不仅包括 参与结缔组织结构(如胶原蛋白)，但也 调节因子，如成分，蛋白酶抑制剂，和 杀菌蛋白质。因为很有可能P. 牙周炎导致中性粒细胞向牙周组织募集 空洞与吞噬酶释放增强异常 结缔组织翻转，调节蛋白的失活 变得更加重要。为了研究潜在的双重角色 更紧密地研究牙龈假单胞菌的蛋白酶，这一研究的具体目的 项目致力于1)它们的分离和表征，2) 确定其特殊性，特别是关于 人工合成底物和天然蛋白质的水解，3) 对其降解炎症和炎症的能力进行评估 抗炎蛋白，以及4)测定其作用 天然蛋白水解酶抑制剂(哺乳动物和细菌 派生)作为潜在的监管者。这项提议的长期目标是 是为了评估牙龈假单胞菌蛋白水解酶对 牙周病的发展，决定这些的结构 酶，并产生抑制物，可以调节它们的活性 活着。

The development of periodontal disease in adults is believed to be associated with the localized secretion of proteinases from the organism Porphyromonas qingivalis into the dento-gingival cavity. These enzymes can degrade a host of important proteins which include not only those involved in connective tissue structure (e.g., collagen) but also regulato factors such as components, proteinase inhibitors, and bactericidal proteins. Since it is likely that the presence of P. gingivalis causes the recruitment of neutrophils to the dento-gingival cavity and the release of phagocytic proteinases which augment abnormal connective tissue turn-over, the inactivation of regulatory proteins becomes even more important. In order to study the potential dual role of P. gingivalis proteinases more closely, the specific aims of this project are dedicated to 1) their isolation and characterization, 2) determination of their specificity, particularly with regard to the hydrolysis of synthetic substrates and naturally occurring proteins, 3) evaluation of their ability to degrade both inflammatory and anti-inflammatory proteins, and 4) measurement of the effect of naturally occurring proteinase inhibitors (mammalian and bacteria derived) as potential regulators. The long term goals of this proposal are to evaluate the contribution of P. gingivalis proteinases to the development of periodontal disease, determine the structure of these enzymes, and produce inhibitors which can regulate their activity in vivo.