ION CHANNELS IN SALIVARY GLAND DUCT CELLS

这些研究的目标是确定离子 支撑唾液腺导管功能的电流和 阐明调节这些电流的细胞内因素。 应用贴片电生理技术的方法 夹紧以补充和扩展使用 放射性示踪技术。推导了要检验的假设。 根据我们初步研究的结果，以及之前 唾液腺腺泡细胞的电生理学研究 一般测试相同的离子电流和电流 腺泡细胞中存在的调节机制也存在于 导管细胞。这项研究利用了全电池电流测量。 来自未经刺激和毒鼠强刺激的HSG-PA组织 培养细胞和切除的单通道电流测量 这些细胞上的膜片。全细胞研究现状 允许对静息细胞膜传导进行分析和表征 以及所涉及的各种胞内因子的影响 在细胞信号转导中，如G蛋白、IP3、钙离子浓度、 蛋白激酶C和钙调蛋白。单通道电流研究 将允许确定钾的特征(和 可能是存在于这些细胞中的氯离子通道。

The goal of these studies is to identify the ionic currents that underlie salivary gland ductal function and to elucidate the intracellular factors that regulate these currents. The approach .Is to use the electrophysiological technique of patch clamping in order to complement and extend findings made using radiotracer techniques. The hypotheses to be tested are derived from results obtained in our preliminary studies, and from previous electrophysiological studies of salivary gland acinar cells, and generally test whether the same ionic currents and current regulating mechanisms present in acinar cells are also present in ductal cells. The studies utilize whole-cell current measurements from unstimulated and muscarinically stimulated HSG-PA tissue culture cells, and single channel current measurements on excised membrane patches from these cells. The whole-cell current studies allow analysis and characterization of resting cell membrane conduc- tances as well as effects of various intracellular agents involved in cell signaling such as G-proteins, IP3, Ca ion concentrations, protein kinase C and calmodulin. The single channel current studies will allow a definitive characterization of the potassium (and possibly the Cl) channels present in these cells.