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LABIAL SALIVARY GLANDS-AUTONOMIC CHARACTERIZATION

唇唾液腺自主特征

基本信息

批准号：
3223088
负责人：
Kenneth T Izutsu
金额：
$ 12.56万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
起止时间：
1991-09-30 至 1994-09-29
项目状态：
已结题

项目摘要

The overall objective of this work is to elucidate the autonomic regulation of human labial gland function. This proposal concentrates on characterizing the autonomic receptors present in the gland, and on documenting some of the biochemical and physiological responses evoked by receptor activation. The biochemical responses of present interest involve changes in gland cyclic nucleotide concentrations, while the physiological responses involve activation of cell K release. The specific aims are as follows: Specific Aim 1. To show that labial glands possess normal numbers of muscarinic cholinergic and beta-adrenergic receptors, and relatively low numbers of alpha-adrenergic receptors. Specific Aim 2. To determine the binding affinities of these receptors for the corresponding agonists and antagonists, and to obtain information regarding the receptor subtypes. Specific Aim 3. To characterize the biochemical and physiological effects of autonomic receptor activation in labial glands. The specific aims dealing with numbers of receptors and receptor binding affinities will be accomplished using a labial gland membrane preparation that will be incubated with appropriate radioligands in the presence or absence of various antagonists. The specific aim dealing with biochemical and physiological effects of autonomic receptor activation will involve incubating labial gland slices in vitro, and measuring receptor induced changes in gland cyclic nucleotide concentrations, or receptor induced activation of gland K release, a measure of gland electrolyte and, presumably, gland fluid secretion. Preliminary results are presented for all aspects of the work and show that the proposed experiments are feasible for labial gland samples. Completion of this work will lead to a significant increase in our knowledge of the normal autonomic regulation of human labial gland secretory function. The data and procedures developed in this study should find numerous applications in future studies in human salivary gland disease.

这项工作的总体目标是阐明自主调节人类唇腺的功能该提案的重点是表征存在于腺体中的自主神经受体，以及记录了一些生物化学和生理反应，受体激活目前感兴趣的生化反应包括腺体环核苷酸浓度的变化，而生理反应涉及细胞K释放的激活。具体目标如下：如下所示：具体目标1。为了证明唇腺具有正常数量的毒蕈碱胆碱能和β-肾上腺素能受体，肾上腺素能受体的数量。具体目标2。为了确定这些受体对以下物质的结合亲和力，相应的激动剂和拮抗剂，并获得信息关于受体亚型。具体目标3。表征生化和生理效应唇腺中自主神经受体的激活。具体目标是处理受体数量和受体结合将使用唇腺膜制备物来完成亲和力其将与适当的放射性配体在存在或缺乏各种拮抗剂。生物化学处理的具体目标和自主神经受体激活的生理效应将涉及体外培养唇腺切片，测定诱导的受体，腺体环核苷酸浓度或受体诱导的变化腺K释放的激活，腺电解质的测量，可能是腺液分泌初步结果，各方面的工作，并表明所提出的实验是可行的唇腺样本这项工作的完成将导致我们的对人类唇腺正常自主调节的认识分泌功能本研究中开发的数据和程序应在人类唾液腺的未来研究中有许多应用疾病