LABIAL SALIVARY GLANDS-AUTONOMIC CHARACTERIZATION

这项工作的总体目标是阐明自主神经调节 人类唇腺的功能。这项建议集中在 表征存在于腺体中的自主神经受体，以及 记录了一些生物化学和生理反应，由 受体激活。目前感兴趣的生化反应包括 腺体环核苷酸浓度的变化，而生理 反应涉及细胞K释放的激活。具体目标如下 以下是： 具体目的1.表明唇腺具有正常数量的 M胆碱能和β-肾上腺素能受体，且相对较低 α肾上腺素能受体的数量。 具体目的2.测定这些受体的结合亲和力 相应的激动剂和拮抗剂，并获得信息 关于受体亚型。 具体目标3.表征生物化学和生理效应 唇腺中自主神经受体的激活。 处理受体数量和受体结合的特定目标 亲和力将使用唇腺膜制剂来完成 它将在存在或存在的情况下与适当的放射性配体孵育 没有不同的对手。处理生化问题的具体目标 而自主神经受体激活的生理效应将涉及 唇腺切片体外孵育及受体诱导量测定 腺体环核苷酸浓度的变化，或受体诱导的 激活腺体释放钾，这是腺体电解质的一个指标， 据推测，是腺液分泌。给出了初步的结果。 所有方面的工作，并表明所提出的实验是可行的 用于唇腺样本。 这项工作的完成将导致我们的 对人体唇腺正常自主神经调节的认识 分泌功能。这项研究中开发的数据和程序应该 在人类唾液腺的未来研究中发现了许多应用 疾病。

The overall objective of this work is to elucidate the autonomic regulation of human labial gland function. This proposal concentrates on characterizing the autonomic receptors present in the gland, and on documenting some of the biochemical and physiological responses evoked by receptor activation. The biochemical responses of present interest involve changes in gland cyclic nucleotide concentrations, while the physiological responses involve activation of cell K release. The specific aims are as follows: Specific Aim 1. To show that labial glands possess normal numbers of muscarinic cholinergic and beta-adrenergic receptors, and relatively low numbers of alpha-adrenergic receptors. Specific Aim 2. To determine the binding affinities of these receptors for the corresponding agonists and antagonists, and to obtain information regarding the receptor subtypes. Specific Aim 3. To characterize the biochemical and physiological effects of autonomic receptor activation in labial glands. The specific aims dealing with numbers of receptors and receptor binding affinities will be accomplished using a labial gland membrane preparation that will be incubated with appropriate radioligands in the presence or absence of various antagonists. The specific aim dealing with biochemical and physiological effects of autonomic receptor activation will involve incubating labial gland slices in vitro, and measuring receptor induced changes in gland cyclic nucleotide concentrations, or receptor induced activation of gland K release, a measure of gland electrolyte and, presumably, gland fluid secretion. Preliminary results are presented for all aspects of the work and show that the proposed experiments are feasible for labial gland samples. Completion of this work will lead to a significant increase in our knowledge of the normal autonomic regulation of human labial gland secretory function. The data and procedures developed in this study should find numerous applications in future studies in human salivary gland disease.