CALCIUM BINDING PROTEINS AND CALCIUM HOMEOSTASIS

维生素D诱导的一个明确和普遍接受的作用 钙结合蛋白(CABP)目前还不可用，尽管 事实上，它的发现已经过去了10多年。结果是 其间几年的研究表明，这种蛋白质参与了 在维生素D依赖的钙代谢中，但是，对确切的 这一作用的机制在很大程度上受到了关于 CABP的免疫细胞学定位。拟议的调查是 旨在系统地调查协议中的变量 在以前的本地化研究中使用，目的是确定 造成不同结果的因素(S)。有了这些信息，一个 将设计可靠的细胞学定位方案。本土化 将在不同的营养和营养条件下对肠道CABP进行研究 已知的生理条件改变肠道钙转运和 CABP含量。CABP将在另外两个版本中使用相同的协议进行本地化 产蛋母鸡的钙转运器官、肾脏和壳腺。这个 蛋白质也会定位在大脑中。无论是光学显微镜还是 CABP的超微结构定位将被研究。可靠的信息 关于CABP在这些组织中的位置(S)将大大有助于确定 蛋白质在钙代谢中的作用(S)，这反过来可能是有价值的 为了更好地了解钙代谢改变的原因 不同的疾病状态。 还提出了一项分离和纯化线粒体钙结合蛋白的研究。 蛋白质，一种糖蛋白(CaBGP)，然后产生针对该蛋白的单特异性抗血清 蛋白。CaBGP的细胞学定位将作为以下函数进行研究 钙化软骨中的矿化状态。潜在的牵涉到 将对CaBGP在钙化反应中的作用进行研究。

A definitive and generally accepted role for the vitamin D-induced calcium-binding protein (CaBP) is not available at the present time despite the fact that more than 10 years have elapsed since its discovery. The results of research in the intervening years have suggested an involvement of the protein in vitamin D-dependent calcium metabolism, but, an understanding of the exact mechanism of that role suffers to a large degree from the controversy concerning the immunocytological localization of CaBP. The proposed investigations are designed to systematically investigate the variables in the protocols which have been used in previous localization studies with the goal of identifying the factor(s) responsible for the divergent results. With that information, a reliable cytological localization protocol will be designed. The localization of intestinal CaBP will be investigated under different nutritional and physiological conditions known to alter both intestinal calcium transport and CaBP content. CaBP will be localized using the same protocol in two other calcium-transporting organs, the kidney and shell gland of the laying hen. The protein will also be localized in brain. Both the light microscopic and the ultrastructural localization of CaBP will be investigated. Reliable information concerning the CaBP site(s) in these tissues will greatly aid in defining the function(s) of the protein in calcium metabolism which in turn may be of value in gaining a better unerstanding of the causes of altered calcium metabolism in different disease states. A study is also proposed to isolate and purify a mitochondrial calcium binding protein, a glycoprotein (CaBGP), then raise a monospecific antiserum against the protein. The cytological localization of CaBGP will be studied as a function of the mineralization state in calcifying cartilage. The potential involvement of CaBGP in calcification reactions will be investigated.