HOST-BACTERIAL INTERACTIONS IN PERIODONTAL DISEASE

牙周炎是一种慢性牙周炎症性疾病。 以疾病活动的爆发和更长的时期为特征 减刑。结扎诱导的牙周炎提供了一个类似的模型 可以加速发病的微生物区系。诱导一种 在正常情况下通过免疫增强免疫反应 建立牙周病病原体(如牙周炎杆菌)可 延缓或减少其殖民，从而影响其发展 和/或牙周炎的严重程度。如果免疫接种能提供一些 保护程度，它将提供更好的理解 牙周炎的发病机制，并可能在 治疗。 这项调查的目的是研究免疫接种的影响。 与优势可培养微生物对宿主细菌的相互作用 在猴子牙周炎模型的进展过程中。具体的 目标是： 1.分离出优势可培养的龈下微生物区系，测定 人群血清和龈沟液中这些细菌抗体水平的研究 用酶联免疫吸附试验检测食蟹猴从牙周炎到牙周炎的进展 结扎术诱导的晚期牙周炎和描述关系 观察到的。 2.检测牙龈类杆菌对猴的免疫效果 通过监测临床、组织学、细菌学从猴子身上分离出 以及结扎诱导的牙周炎期间的体液反应。会是 确定牙龈假单胞菌是否定植，患病率 疾病的进展和/或程度是从一组 假免疫、结扎的猴子和一组免疫的牙龈假单胞菌 未结扎的猴子。

Periodontitis is a chronic inflammatory disease of the periodontium characterized by bursts of disease activity and longer periods of remission. Ligature-induced periodontitis provides a model with a similar microflora in which the rate of disease can be accelerated. Induction of a heightened immune response via immunization prior to the usual establishment of a periodontopathic organism (such as B. gingivalis) may delay or reduce its colonization and thereby influence the development and/or severity of periodontitis. If immunization could provide some degree of protection, it would provide a better understanding of the pathogenesis of periodontitis and would potentially be of value in treatment. The purpose of this investigation is to study the influence of immunization with a predominant cultivable microorganism on host bacterial interactions during the progression to periodontitis in a monkey model. The specific aims are to: 1. Isolate the predominant cultivable subgingival microflora, determine the serum and gingival crevicular fluid antibody levels to these organisms in cynomolgus monkeys by ELISA during the progression from gingivitis to ligature-induced, advanced periodontitis and describe relationships observed. 2. Examine the efficacy of monkey immunization with Bacteroides gingivalis isolated from monkeys by monitoring the clinical, histological, bacterial and humoral responses during ligature-induced periodontitis. It will be determined if the colonization by B. gingivalis, the rate of disease progression and/or the extent is of disease is modified from a group of sham-immunized, ligated monkeys and a group of B. gingivalis immunized non-ligated monkeys.