CARBOHYDRATE AND LIPID METABOLIC PATHWAYS

方法将被开发并应用于定量的途径 碳水化合物和脂肪代谢。使用分裂的猴脑制剂，一种 研究胰岛素对下丘脑葡萄糖代谢的影响。 从C14特别标记的脂肪酸的C14的命运来看， α-和欧米伽氧化对脂肪酸代谢的贡献将是 在饥饿和糖尿病期间，在肌肉、肝脏和大脑中测定。 这些组织中酮体的碳的命运也将被评估。 包括它们可能对糖异生的中间体的贡献。 来自特定标记脂肪酸的C14O2的产量将被用作衡量标准 脂肪酸氧化过程中游离中间体的形成。的来源 胎盘中的果糖将通过追踪C14-和C14的形成来测量 H3标记的葡萄糖。为此，多元醇的酶的立体特异性 关于还原核苷酸形成的途径将被确定。

Methods will be developed and applied to the quantitation of pathways of carbohydrate and lipid metabolism. Using a split monkey brain preparation, an effect of insulin on glucose metabolism by the hypothalamus will be sought. From the fate of C14 of specifically C14-labeled fatty acids, the extent that alpha- and omega- oxidation contribute to fatty acid metabolism will be determined in muscle, liver, and brain during starvation and diabetes mellitus. The fate of the carbons of ketone bodies in these tissues will also be assessed including their possible contribution of intermediates for gluconeogenesis. Yields of C14O2 from specifically labeled fatty acids will be used as a measure of formation of free intermediates in fatty acid oxidation. The source of fructose in placenta will be measured by tracing its formation from C14- and H3-labeled glucose. For this the stereospecificity of the enzymes of the polyol pathway in regard to reduced nucleotide formation will be determined.