INSULIN AND HGH: SECRETION AND METABOLIC ACTIONS

我们将描述胰岛素样生长因子IGF I的分泌 和IGF II，它们与血清蛋白的结合及其与 在正常生长和发育期间的特定受体，以及在 各种相关的病理状态。我们已经确定了一个 一整组患有孤立的IGF I缺陷的个体， 以前未知的人类生长迟缓的原因。通过组合数据 胰岛素样生长因子及其载体状态与生长激素的关系 分泌，我们将在重新定义增长原因方面取得重大进展 智力迟缓。更具体地说，性行为的整个分类 遗传性侏儒症将利用特定生长的数据重建 各种因素。这将提供更好的理解和更多的 治疗生长发育问题的基本原理。的影响 饥饿、饮食结构和重要激素(雌激素、 睾酮、生长激素和人胎盘催乳素)对IGF分泌的影响 而IGF与血清蛋白的结合将在体内和体外进行检测。在……里面 体外研究将涉及至少三种特定的组织，其中一种能够 产生IGF I和II(分离的肝细胞)另一种(真皮) 成纤维细胞)仅产生IGF-I。胰岛素样生长因子与心理健康的关系 将对糖尿病并发症进行调查。我们已经做出了值得注意的 10多年来对这一领域的贡献。我们目前的初步调查 数据(唯一可用的)表明血清蛋白与胰岛素样生长因子结合减少 在糖尿病受试者中--这可能是理解 生长激素与本病并发症的关系。这个 有能力通过IGF I和IFG II的特定分析来实现我们的目标 代表着我们实验室的重大发展进步。致老年人 据调查人员所知，美国没有一个单独的实验室 具有可比能力的国家。

We will characterize the secretion of insulin-like growth factors, IGF I and IGF II, their binding by serum proteins and their reactions with specific receptors during both normal growth and development, and in a variety of relevant patholigical states. We have already identified an entire group of individuals with an isolated deficiency of IGF I, a previously unknown cause of growth retardation in man. By combining data on IGF and its carrier state with that obtained on growth hormone (GH) secretion, we will make a major advance in redefining the causes of growth retardation. More specificially, the entire classification of sexual ateliotic dwarfism will be reconstructed utilizing data on specific growth factors. This will provide for both a superior understanding and a more rationale approach to therapy of growth problems. The effect of starvation, diet composition and important hormones (estrogen, testosterone, growth hormone and human placental lactogen) on IGF secretion and IGF binding by serum protein will be examined in vivo and in vitro. In vitro studies will involve at least three specific tissues, one capable of producing IGF I and II (the isolated hepatocyte) another (dermal fibroblast) producing IGF I only. The relationship of IGF to the complications of diabetes will be investigated. We have made noteworthy contributions to this field for over 10 years. Our current preliminary data (the only available) indicate decreased IGF binding by serum proteins in diabetic subjects -- a possible important "link" in understanding the relationship of growth hormone to the complications of this disease. The ability to obtain our objectives by specific assays for IGF I and IFG II represents a major developmental advance for our laboratory. To the Senior Investigator's knowledge, there is no single laboratory in the United States with a comparable capacity.