KIDNEY TRANSPLANTATION AND DONOR-SPECIFIC TRANSFUSIONS

术前输血(随机或随机输血)的益处 对肾移植(身体或活体)存活率的影响 相关的)已经被很好地记录下来。然而，机制(S)尚不清楚 尽管进行了广泛的研究。我们将使用定义明确的捐赠者特定的 安徽省部分亲属活体组合的输血模式 试图找出促成这一惊人成功的机制 这种型号的。我们希望所获得的信息能够扩大到 随机输血随机输血的情况这是有计划的 详细研究供者特异性的6种潜在机制 输液系统。1)选择高响应者和低响应者，2) 诱导无反应性，3)特异性和非特异性的激活 抑制细胞，4)抗体的诱导，5)免疫调节 6)原位异体移植物改变。体外测试系统，如 MLC、CML、PLT、免疫荧光、人类白细胞抗原血清学和监测 将使用带有单抗的淋巴细胞亚群。此外, 原位同种异体移植反应将使用细针进行研究。 利用最新发展的标记技术的吸液细胞学 抗人类白细胞抗原-A、B、C、DR和 β-2抗血清。从这些实验中获得的信息将是 用于优化献血者特定的输血方案 试图降低敏感度，确定其有效性 输血期间的免疫抑制，以及 此外，还得出了关于随机供体机制的结论 输血。

The beneficial effect of preoperative blood transfusions (random or donor-specific) on the survival of renal transplants (cadaver or living related) has been well documented. The mechanism(s) however are unknown despite extensive research. We will use the well defined donor-specific blood transfusion model in selected living related combinations in an attempt to identify mechanisms which contribute to the spectacular success of this model. We hope that the information obtained can be extended to the situation in which random blood transfusions are given. It is planned to investigate in detail 6 potential mechanisms in the donor-specific transfusion system. 1) Selection into high and low responders, 2) induction of unresponsiveness, 3) activation of specific and non-specific suppressor cells, 4) induction of antibodies, 5) immunomodulation of the recipient, and 6) in situ allograft changes. In vitro test systems such as MLC, CML, PLT, immunofluorescence, HLA serology, and monitoring of lymp]hocyte subsets with monoclonal antibodies will be used. In addition, the in situ allograft reaction will be investigated using fine needle aspiration cytology utilizing recently developed techniques of labelling cytocentrifuge preparations with monoclonal and anti-HLA-A, B, C, DR and Beta-2 antisera. The information gained from these experiments will be utilized to optimize the donor-specific blood transfusion protocol in an attempt to decrease sensitization, determine the effectiveness of immunosuppression during the administration of blood transfusions, and additionally come to conclusions about the mechanisms of random donor transfusions.