TRANSITIONS AND TRANSPORT IN GASTRIC MUCOSA

这项工作的主要目标是确定酸分泌是如何 兔胃壁细胞维持离子内稳态 它们的分泌活动发生了巨大的变化。微量荧光分光光度法和 PH、Na-、Ca-、Cl-和K敏感荧光的数字图像处理 染料将被用来确定离子交换剂(Na/H、Cl/HCO3和 Na/Ca)、共转运蛋白(Na/HCO3)、三磷酸腺苷酶(Na/K和H/K)和钙 “通道”由H2激动剂组胺和毒扁豆碱控制。 激动剂卡巴胆碱。不同的第二使者像Ca， PH、cAMP和蛋白激酶C可能在刺激分泌偶联意志中发挥作用 还研究了Na/H和Cl/HC03交换的作用 可能会在维护 分泌酶的主细胞和分泌粘液的表面上皮细胞。 这些数据将对了解胃细胞如何在体内发挥作用很重要。 健康和患病的状态。此外，由于膜的运输过程 刺激-分泌耦合机制存在于大多数分泌型 细胞，这项工作应该有相当广泛的相关性。

The major goal of this work is to determine how the acid-secreting parietal cells of the rabbit stomach maintain ionic homeostasis during large changes in their secretory activity. Microspectrofluorimetry and digital image processing of pH-, Na- Ca-, Cl- and K-sensitive fluorescent dyes will be used to determine how the ion exchangers (Na/H, Cl/HCO3, and Na/Ca), cotransporter (Na/HCO3), ATP-ases (Na/K and H/K), and Ca "channels" are controlled by the H2 agonist histamine and muscarinic agonist carbachol. The roles which different second messengers like Ca, pH, CAMP and protein kinase C may play in stimulus secretion coupling will also be investigated, as will the roles which Na/H and Cl/HC03 exchange might play in maintenance of the "barrier" function of the enzyme-secreting chief cells and mucus-secreting surface epithelial cells. These data will be of importance in knowing how stomach cells operate in healthy and diseased states. Also, since the membrane transport processes and stimulus-secretion coupling mechanisms are present in most secretory cells, the work should have quite broad relevance.