CELLULAR PD AND ION ACTIVITIES IN RENAL PROXIMAL TUBULES

这个项目的主要目标是更详细地研究 这些调节因素之间高度相互依赖的关系 细胞内钠的活性与钠的净转运和转运 氢离子和钾离子的体系。孤立灌流近端 曲直小管段和近端直小管段 大脑皮层在运输和细胞调节方面将进行比较 功能。拟议实验的具体目的是：1) 检测胞外和胞内pH对阴离子和 管腔和基侧膜的阳离子渗透选择性2) 调查导致这种差异的潜在机制 底侧膜的表观碳酸盐电导 近端曲管和近端直管3)检查现象 曲管中的电势振荡相对于 PH和4的转运和细胞调节之间的相互作用 重点研究细胞内钾的调节 在管腔膜上。测量将用常规方法进行 微电极和双桶状离子选择电极用于 钠、钾、氯化物和氢气。长期目标是 阐明细胞离子交换和肾脏的基本机制 运输过程维持电解质和水的动态平衡控制 体液平衡。

The principal aims of this project are to examine in greater detail the highly interdependent relationships between those factors which regulate intracellular sodium activity and net sodium transport and the transport systems for hydrogen and potassium ions. Isolated perfused proximal convoluted and proximal straight tubule segments from the superficial cortex will be compared with regard to transport and cell regulatory functions. The specific aims of the proposed experiments are to: 1) examine the effect of extracellular and intracellular pH on anion and cation perm-selectivity of the luminal and basolateral membranes 2) investigate the underlying mechanisms which result in the difference in apparent bicarbonate conductance of the basolateral membrane between the proximal convoluted and proximal straight tubule 3) examine the phenomenon of electrical potential oscillations in convoluted tubules with respect to the interaction between transport and cellular regulation of pH and 4) investigate the regulation of cellular potassium with particular emphasis on the luminal membrane. Measurements will be made with conventional microelectrodes and with double barreled ion selective electrodes for sodium, potassium, chloride, and hydrogen. The long term goals are to elucidate the basic mechanisms by which cellular ion exchanges and renal transport processes maintain homeostatic control of electrolyte and water balance of the body fluids.