KIDNEY TRANSPLANTATION AND DONOR-SPECIFIC TRANSFUSIONS

术前输血的有益效果（随机或 供体特异性）对肾移植存活率（尸体或活体 相关的）都有很好的记录。 然而，机制尚不清楚 尽管进行了广泛的研究。 我们将使用定义明确的捐赠者专用 输血模型在选定的生活相关的组合， 试图找出有助于取得巨大成功的机制， 这个模型。 我们希望所获得的资料可以扩大到 随机输血的情况。 计划 详细研究供体特异性的6种潜在机制， 输血系统 1)选择高应答者和低应答者，2） 诱导无反应性，3）特异性和非特异性激活 抑制细胞，4）诱导抗体，5）免疫调节细胞， 6）原位移植物改变。 体外测试系统，例如 MLC、CML、PLT、免疫荧光、HLA血清学和监测 将使用具有单克隆抗体的淋巴细胞亚群。 此外，本发明还提供了一种方法， 将使用细针研究原位同种异体移植反应 利用最近开发的标记技术的抽吸细胞学 用单克隆抗体和抗HLA-A、B、C、DR和 β-2抗血清。 从这些实验中获得的信息将是 用于优化供体特异性输血方案， 尝试降低致敏性，确定 输血期间的免疫抑制，以及 并对随机供体的作用机理进行了探讨 输血

The beneficial effect of preoperative blood transfusions (random or donor-specific) on the survival of renal transplants (cadaver or living related) has been well documented. The mechanism(s) however are unknown despite extensive research. We will use the well defined donor-specific blood transfusion model in selected living related combinations in an attempt to identify mechanisms which contribute to the spectacular success of this model. We hope that the information obtained can be extended to the situation in which random blood transfusions are given. It is planned to investigate in detail 6 potential mechanisms in the donor-specific transfusion system. 1) Selection into high and low responders, 2) induction of unresponsiveness, 3) activation of specific and non-specific suppressor cells, 4) induction of antibodies, 5) immunomodulation of the recipient, and 6) in situ allograft changes. In vitro test systems such as MLC, CML, PLT, immunofluorescence, HLA serology, and monitoring of lymp]hocyte subsets with monoclonal antibodies will be used. In addition, the in situ allograft reaction will be investigated using fine needle aspiration cytology utilizing recently developed techniques of labelling cytocentrifuge preparations with monoclonal and anti-HLA-A, B, C, DR and Beta-2 antisera. The information gained from these experiments will be utilized to optimize the donor-specific blood transfusion protocol in an attempt to decrease sensitization, determine the effectiveness of immunosuppression during the administration of blood transfusions, and additionally come to conclusions about the mechanisms of random donor transfusions.