CONVERSION OF ORNITHINE, ARGININE, PROLINE AND GLUTAMATE

合成氨甲酰的酶和代谢途径 磷酸盐将主要在哺乳动物中进行研究。 研究的目的是 了解途径的调节，即精氨酸（和尿素）和UMP 生物合成 希望这种尿素生物合成的研究将是 用于急性氨中毒。 在UMP生物合成的情况下 很明显，这些酶的水平变化很大，因为这两个途径， 分化和发育，使组织迅速分裂，如 癌症、再生和正常肝脏、大脑等，这些物质的含量很高 内切酶 我们还想看看这些酶是否可以被“诱导”， “抑制”，或者如果增加的水平仅仅是酶的结果， 稳定化 研究将涉及分离的蛋白质的纯化和动力学表征。 两种途径的酶或UMP途径的酶复合物，以及研究 在生长期间这些哺乳动物酶的体内代谢控制上， 分化，或者当营养或激素状态发生变化时， 正常个体以及异常组织的研究，如癌症或 突变细胞 粪链球菌天冬氨酸转氨甲酰酶的研究进展 弗劳地柠檬酸杆菌可能会继续，以及对激活剂的工作， 酵母碳酸酐酶

The enzymes which synthesize and the metabolic pathways which utilize carbamyl phosphate will be studied principally in mammals. The aim of the studies is to understand the regulation of the pathways, namely, arginine (and urea) and UMP biosynthesis. It is hoped that such studies for urea biosynthesis will be useful in acute ammonia intoxication. In the case of the UMP biosynthetic pathway, it is clear that the levels of these enzymes vary greatly due both to differentiation and development, so that tissues which divide rapidly such as cancers, regenerating and normal liver, brain, etc., have high levels of these enzymes. We would also like to see if the enzymes can be "induced" and "repressed," or if increased levels are merely the result for enzyme stabilization. Studies will involve purification and kinetic characterization of isolated enzymes of both pathways or the enzyme complexes of the UMP pathway, and studies on the in vivo metabolic control of these mammalian enzymes during growth, differentiation, or when changes in nutritional or hormonal states occur in normal individual as well as studies of abnormal tissues such as cancer or mutant cells. Former work on the aspartate transcarbamylases of Streptococcus faecalis and Citrobacter freundii may be continued, as well as work on the activator of the yeast carbonic anhydrase.