A quantitative colorimetric assay for semialdehydes.
A quantitative colorimetric assay for semialdehydes.
复制标题
半醛的定量比色测定。
DOI：
10.1016/0003-2697(90)90272-b
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发表时间：
1990
影响因子：
2.9
通讯作者：
Jones,ME
中科院分区：
文献类型：
--
作者：
Small,WC;Jones,ME
We have studied the ability of the enzyme pyrroline-5-carboxylate dehydrogenase to oxidize aldehydes which are structurally similar to pyrroline-5-carboxylate, the enamine of γ-glutamyl semialdehyde. These studies required the organic synthesis of semialdehydes not commercially available. Accurate quantitation of the concentration of these compounds in solution is necessary because they polymerize upon evaporation to dryness. Reported here is a method for colorimetric measurement of semialdehydes in solution by formation of an oxidized covalent adduct with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole. This adduct after oxidation produces a product that has an absorption maximum at 302 ± 2 nm. This characteristic absorbance maximum was not found in the spectra of the covalent adducts of simple aliphatic aldehydes with the chromophore. Using glyoxylic acid as standard, the values obtained for the concentration of semialdehydes in solution were independently verified from quantitation of the proton concentration of the oxidized adducts by integration of the area under the peaks of their1H NMR signals. These latter values were compared to the signal strengths of an internal standard. The values obtained by the colorimetric and NMR techniques were in excellent agreement, thus allowing use of the colorimetric technique as a simple method for routine laboratory analysis of semialdehydes.
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L-吡咯啉-5-羧酸的酶法合成和纯化。
影响因子：
2.9
作者：
R. Smith;S. Downing;J. Phang
通讯作者：
J. Phang
D-氨基酸氧化酶和尿酸氧化酶活性的新比色测定。
影响因子：
2.9
作者：
T. Watanabe;Y. Motomura;T. Suga
通讯作者：
T. Suga
影响因子：
3
作者：
H. Durst;G. Gokel
通讯作者：
G. Gokel
使用 4-氨基-3-肼基-5-巯基-1,2,4-三唑和尿酸氧化酶-过氧化氢酶系统测定尿酸
影响因子：
1.7
作者：
Takafumi Watanabe;T. Suga
通讯作者：
T. Suga
DOI：
10.1016/s0021-9258(18)65530-9
发表时间：
1954
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Hendler;C. Anfinsen
通讯作者：
C. Anfinsen