NEURAL CONTROL OF HUMAN INTESTINAL MUCOSA

提出了一种检验本征神经的实验方案。 影响人类大肠粘膜功能的机制。这个 该项目的总体目标有三个方面。一是建立基础 离子吸收和分泌速率及其机制的研究 参与这些运输过程的人。二是分析 肠神经的激活对结肠粘膜功能的影响； 三是确定肠神经和肠神经的类型 神经递质类物质，介导神经元对大脑皮层的影响 肠粘膜。这些研究将在平板剂制剂上进行 体外手术切除人大肠。基本方法 将组织安装在Ussing类型的流量室中，这些流量室经过修改 用于电场刺激壁内神经元，并测量和 评估引起的短路电流和离子通量的变化 或通过电激活壁内神经进行修饰。药理作用 对下列可能的神经递质物质进行分析和检测 通过电场刺激释放到器官浴中的是 识别影响神经功能的神经类型的主要方法 粘膜。该项目将绕过动物模型，并提供明确的 肠道神经系统之间功能关系的信息 以及人类大肠的粘膜上皮。预计它将 提供有关改进所需的基本功能的新信息 对粘膜异常分泌和吸收过程的认识 这是人类便秘和腹泻状态的基础。

An experimental plan is proposed for examining the intrinsic neural mechanisms that influence mucosal functions in human large intestine. The overall aim of the project is threefold. The first is to establish basal rates of absorption and secretion of ions and to investigate the mechanisms that are involved in these transport processes. The second is to analyze the effects of activation of enteric nerves on colonic mucosal function; and the third is to identify the types of enteric nerves and neurotransmitter substances that mediate neuronal influences on the large intestinal mucosa. The studies will be done on flat sheet preparations of surgically resected human large intestine in vitro. The basic approach will be to mount the tissues in Ussing type flux chambers that are modified for electrical field stimulation of intramural neurons and to measure and evaluate changes in short-circuit currents and ionic fluxes that are evoked or modified by electrical activation of intramural nerves. Pharmacological analysis and assays for putative neurotransmitter substances that are released into the organ bath by electrical field stimulation will be the principal methods for identifying the types of nerves that influence the mucosa. The project will bypass animal models and provide definitive information on functional relationships between the enteric nervous system and the mucosal epithelium in the human large intestine. It is expected to yield new information on basic function that is needed for better understanding of the abnormal secretory and absorptive mucosal processes that underlie constipation and diarrheal states in humans.