NEURAL REGULATION OF INTESTINAL MUCOSA

这项研究旨在检查内在的神经机制， 对豚鼠回肠黏膜分泌功能的影响。 第一个目标是确定影响粘膜的神经递质。 在粘膜下神经丛的突触回路中起作用，并在 神经上皮连接。第二个目标是识别神经递质。 参与影响肠道离子的反射通路 当葡萄糖从粘膜缓冲液中移除时的转运过程 解决办法。这些研究将在几内亚的平板制剂上进行。 钝化切除外肌的猪回肠 解剖。豚鼠回肠是最好的研究动物模型 肠神经的类型决定了肠粘膜功能的神经调节 在粘膜下神经丛内已被发现，并且 肠系膜神经元的电生理特性已有报道。 这些组织将被安装在Ussing助焊剂室中，这些助熔剂室被改装成 电场刺激对壁内神经元的影响 在电气过程中将测量短路电流和离子通量 肠神经的激活。标识的主要方法 影响粘膜的神经类型将是药理学分析 以及释放到浴缸中的假定神经递质的检测 在电场刺激过程中。拟议的研究将扩大 对豚鼠体内离子转运过程的了解非常有限 控制小肠运输的神经生理机制 功能。它将提供有关反射通路的新信息，这些信息可能 控制肠道内容物在正常功能过程中的流动性 肠道，或在分泌性腹泻等病理生理状态期间。

This study is designed to examine intrinsic neural mechanisms that influence intestinal mucosal secretory function in the guinea pig ileum. The first objective is to identify neurotransmitters that influence mucosal function within the synaptic circuitry of the submucosal plexus, and at neuroepithelial junctions. The second aim is to identify neurotransmitters that are involved in a reflex pathway that influences intestinal ion transport processes when glucose is removed from the mucosal buffer solutions. The studies will be done on flat sheet preparations of guinea pig ileum that have had the muscularis externa removed by blunt dissection. The guinea pig ileum is the best animal model for studying neural regulation of mucosal function because the types of enteric nerves within the submucosal plexus have been identified, and the electrophysiological properties of the enteric neurons have been reported. The tissues will be mounted in Ussing flux chambers that are modified for electrical field stimulation of intramural neurons, and changes in short-circuit current and ion fluxes will be measured during electrical activation of enteric nerves. The principal methods for identifying the types of nerves that influence the mucosa will be pharmacological analyses and assays for putative neurotransmitters that are released into the bath during electrical field stimulation. The proposed study will expand the very limited knowledge of ion transport processes in the guinea pig and of the neurophysiological mechanisms that control small intestinal transport function. It will provide new information on reflex pathways that may govern the fluidity of the intestinal contents during normal functioning of the bowel, or during pathophysiological states such as secretory diarrhea.