New Technology for High-Performance and High-Capacity Bio-separations: Protein Purification using "Hydrophobic Water" Solvent Systems

摘要：在生物技术行业中，高价值蛋白质、多肽和生物分子的生产需要快速、低成本、可靠、符合gmp和可扩展的生产方法。用于实现这一目标的一个科学领域是使用预备色谱法和相关的提取技术。建议采用三种相对较新的技术组合，即：(1)离子液体；(2)现代高效逆流色谱（HPCCC）和离心分配色谱（CPC）；(3)无共溶剂、由阳离子表面活性剂衍生的高含水量微乳液。所提出的微乳液溶剂体系是一类具有表面活性的疏水离子液体，可用于多肽、蛋白质和其他生物分子的高效、高容量和环保分离。在这些分离中，移动相或载体相通常是水，这使得该过程成本低且对环境无害。这是一个高度跨学科的项目，它将结合生物科学、生物化学、化学工程、物理和合成化学的元素。本项目将利用离子液体溶剂系统和微乳液溶剂系统进行具有商业意义的多肽和蛋白质的溶剂萃取和逆流色谱制备、纯化和分离。这将首先使用蛋白质的模型分离，开发和整合这种新方法的不同技术部分，然后使用这些信息从天然来源进行蛋白质混合物的分离。此外，该项目旨在通过使用安装在QUILL研究中心的高容量CPC设备来扩大蛋白质分离，并采用自动化来降低分离的运行成本。该项目的最终目标是生产一种通用的、清洁的、水基的、高效的、具有成本效益的肽和蛋白质分离系统，可以被英国生物技术产业利用，并帮助英国经济中这些部门的增长。

New Technology for High-Performance and High-Capacity Bio-separations: Protein Purification using "Hydrophobic Water" Solvent Systems in Countercurrent Chromatography ApparatusSummaryIn the biotechnology industries, the manufacture of high-value proteins, peptides and biomolecules require fast, low cost, reliable, GMP-compliant and scalable production methodologies. One scientific area used to achieve this, is the use of preparatory chromatography and related extraction technologies. It is proposed to use a combination of three relatively new technologies, namely: (1) ionic liquids, (2) modern high performance countercurrent chromatography (HPCCC) and centrifugal partition chromatography (CPC) and (3) co-solvent free, high water content microemulsions derived from catanionic surfactants. The microemulsion solvent systems proposed are a class of surface active, hydrophobic ionic liquids, which enable a new range of high performance, high capacity and environmentally friendly separations of peptides, proteins and other biomolecules. The mobile or carrier phase in these separations is usually water, which makes the process cheap and environmentally benign. This is a highly multidisciplinary project, which will combine elements of bioscience, biochemistry, chemical engineering, physical, and synthetic chemistry. This project will make use of ionic liquids solvent systems and microemulsion solvent systems in the solvent extraction and countercurrent chromatographic preparation, purification and isolation of peptides and proteins of commercial importance. This will initially use model separations of proteins, to develop and integrate the different technological parts of this new methodology, and then use this information to carry out separations of proteins mixtures from natural sources. Also, this project aims to scale up the protein separations by the use of high capacity CPC equipment installed in the QUILL Research Centre and employ automation to reduce the running costs of the separations. The ultimate goal of this project is to produce a general purpose, clean, water based, efficient, cost effective peptide and protein separation system, that can be exploited by the UK biotechnology industry and help in the growth of these sectors in the UK economy.