ANIMAL MODEL OF INTERSTITIAL CYSTITIS

这项提案的总体目标是建立猫咪泌尿系统综合征 (FUS)作为人类间质性膀胱炎(IC)的动物模型。 为了实现这一目标，我们将研究具有自然产生的FU的猫 属于客户，并在我们的研究猫咪养殖场饲养。 间质性膀胱炎是一种疼痛、虚弱的疾病，通常 折磨着女性。对……的理解上的重大障碍 IC的原因是缺乏一种自然发生的动物模型 疾病。有FUS的母猫几乎符合所有客观标准 LC的诊断。此外，FUS的迹象似乎比 ICDO的症状，这一特征可以通过检查猫来利用 无论是当他们出现症状时，还是当他们“缓解”到 当动物看起来正常时，确定出现了哪些测压计。它 假设感觉神经元和/或肥大细胞(MC)在 在FUS和IC中有重要作用，但活检前膀胱扩张 样本采集通过以下方式抑制P物质(SP)和MC鉴定 诱导感觉神经元MC和SP释放组胺。另外， 糖胺多聚糖(GAG)排泄减少和膀胱增加 患有FUS的猫存在通透性。这些异常也有 在女性身上发现，允许使用猫来检查它们在 IC的发病机制。 这项为期三年的建议有四个主要目标：(一)建立 用NIDDK IC标准诊断母猫的FUS，并进行比较 数据收集自正常母猫和非FUS尿液猫 肠道疾病。所有猫都将被重新检查，如果再次出现口臭的迹象，或在 如果他们不这样做，那就是一年。根据之前的研究，我们预计一年 复发率约为50%。这些动物将不会被评估 更频繁地避免重复膀胱的潜在治疗效果 疾病过程中的肿胀。(2)检查膨胀的影响 膀胱壁SP和MC的表达。(3)确定FUS对 尿液中的糖胺聚糖排泄。(4)确定FUS的影响 99mTcDTPA对膀胱通透性的研究 同时使用客户拥有的猫和研究猫是 这些调查是因为它们会让我们研究一种自然 LC发病模型的建立。

The overall goal of this proposal is to establish Feline Urologic Syndrome (FUS) in female cats as a model of Interstitial Cystitis (IC) in humans. To accomplish this goal, we will study cats with naturally-occurring FUS belonging to clients, and cats maintained in our research cattery. Interstitial cystitis is a painful, debilitating disease that usually afflicts women. A significant impediment to progress in understanding of the causes of IC is the lack of a naturally-occurring animal model of the disease. Female cats with FUS meet nearly all the objective criteria for diagnosis of lC. Moreover, signs of FUS appear to wax and wane more than the symptoms of IC do, a feature that can be exploited by examining cats both when they are showing signs and when they "go into remission" to determine which palimeters are present when the animal appears normal. It is hypothesized that sensory neurons and/or mast cells (MC) play a significant role in FUS and IC, but bladder distention prior to biopsy sample collection inhibits Substance P (SP) and MC identification by inducing release of histamine from MC and SP from sensory neurons. Also, decreased glycosaminoglycan (GAG) excretion and increased bladder permeability are present in cats with FUS. These abnormalities also have been found in women, permitting use of cats to examine their role in the pathogenesis of IC. This three year proposal has four major objectives: (1) To establish the diagnosis of FUS in female cats by NIDDK IC criteria, and to compare these with data collected from normal female cats, and cats with non-FUS urinary tract disease. All cats will be re-examined if signs of FUS recur, or at one year if they do not. Based on previous studies, we expect a one-year recurrence rate of approximately 50%. These animals will not be evaluated more frequently to avoid potential therapeutic effects of repeated bladder distention on the disease process. (2) To examine the effect of distention on SP and MC in bladder wall. (3) To determine the effects of FUS on glycosaminoglycan excretion in urine. (4) To determine the effects of FUS on bladder permeability using 99mTcDTPA. The use of both client-owned and research cats is an important aspect of these investigations because they will allow us to study a naturally occurring disease model of lC.