SECRETORY IGA SYSTEM OF THE EYE

这项拨款申请的长期目标是 我们对眼睛粘膜免疫的理解。 提出 研究的重点是大鼠的反应和功能， 眼分泌免疫系统，重点放在 衰老和营养对眼睛免疫活性的影响。 实验方法包括ELISA。 RIA，ELISP 0 T测定， 免疫荧光，细胞分离和培养，杂交瘤 生产、流式细胞术和放射自显影。 具体目标是： 1)目的：明确泪液中IgA细胞的来源， 2）评价非侵入性眼内免疫反应 可溶性和颗粒性抗原。 这些研究包括审查： a）处理局部应用于眼表面的抗原; B）引发泪液IgA的各种抗原暴露途径，和 c）抗原剂量对泪液抗体应答的影响; d）用抗原胃肠外引发或加强对泪液的影响 抗体水平; e）免疫记忆的潜力， 眼分泌性免疫系统;和f）分泌性免疫系统的比较， 眼内的免疫反应与其他粘膜分泌物中的免疫反应不同。 3)分析眼分泌功能反应， 免疫系统抵抗病毒感染 这项研究包括 确定：a）病毒侵入泪腺对 泪液伊加、IgG、分泌成分（SC）和总蛋白水平; B）病毒感染对含有Ig的细胞密度的影响 泪腺组织和腺泡中的细胞和淋巴样聚集体 SC和Ia抗原的细胞表达; 对病毒感染的抗体反应; d）病毒侵入的影响 对眼粘膜对非侵入性抗原的应答的影响; 在随后的眼部接种活的或灭活的病毒 对病毒攻击的应答;和f）伊加的功能作用 抗病毒抗体在眼部防御中的作用 4)以确定 增龄和营养对眼分泌性免疫的影响 a）老化对眼睛的影响 对非侵入性和病毒抗原的分泌性免疫应答; B） 重度蛋白质营养不良对泪液伊加、IgG水平影响 及SC和泪腺含免疫球蛋白的含量 c）蛋白质营养不良和衰老对细胞的影响 对非侵入性和病毒抗原的眼部免疫应答。 这些 研究与眼睛健康相关：它们解决了 分泌免疫系统的功能作用，它作为 第一道防线，以保护眼表， 细菌定植和病毒入侵。

The long-range objective os this grant application is to advance our understanding of mucosal immmunity in the eye. Proposed research focuses upon the response and function of the rat ocular secretory immune system, with emphasis placed upon the impact of aging and nutrition on immunological activity in the eye. Experimental methods include ELISAs. RlAs, ELISPOT assays, immunofluorescence, cell isolation and culture, hybridoma production, flow cytometry and autoradiography. Specific aims are: 1) To identify the sources of IgA-containing cells in the lacrimal gland; 2) To evaluate the ocular immune response to non-invasive soluble and particulate antigens. These studies involve examining: a) processing of topically applied antigen to the ocular surface; b) various routes of antigenic exposure for eliciting tear IgA and IgG; c) influence of antigen dosage on tear antibody responses; d) impact of parenteral priming or boosting with antigen on tear antibody levels; e) potential for immunological memory in the ocular secretory immune system; and f) comparison of the secretory immune response in the eye to that in other mucosal secretions. 3) To analyze the functional response of the ocular secretory immune system to viral infection. This research includes determining: a) impact of viral invasion in the lacrimal gland on tear IgA, IgG, secretory component (SC) and total protein levels; b) influence of viral infection on the density of Ig-containing cells and lymphoid aggregates in lacrimal tissue and the acinar cell expression of SC and la antigens; c) tear and cellular antibody response to viral infection; d) effect of viral invasion on ocular mucosal responses to non-invasive antigens; e) influence of immunization with live or inactivated virus on subsequent ocular response to viral challenge; and f) functional role of IgA antibodies to viral antigens in ocular defense. 4) To determine the effect of aging and nutrition on the ocular secretory immune system by assessing the a) influence of aging on the ocular secretory immune response to noninvasive and viral antigens; b) effect of severe protein malnutrition on tear levels of IgA, IgG and SC and lacrimal gland content of immunoglobulin-containing cells; and c) impact of protein malnutrition and senescence on the ocular immune response to non-invasive and viral antigens. These studies have health-relatedness for the eye: they address the functional role of the secretory immune system, which serves as the first line of defense to protect the ocular surface against bacterial colonization and viral invasion.