EFFECTS OF REPRODUCTIVE TOXICANTS ON TESTICULAR CELLS

生殖系统是许多广泛传播的化学物质的目标。 家庭、农业或工业用途。而毒性的作用机制 在大多数情况下是未知的，很明显， 男性对二溴氯丙烷、烷基化合物等化学物质敏感 乙二醇醚和某些邻苯二甲酸酯。对其机理的研究 的复杂性阻碍了生殖毒物的使用。 精子发生的生理生化过程。然而， 睾丸的功能单位是生精小管，它是 相对容易分离和培养其原代细胞类型。这 将使用简化的系统来检查这些化学品的影响 以上是在精心控制的组织培养条件下列出的。 简而言之，目标是：(1)确定其毒性机制 三组生殖毒物使用的离体睾丸 细胞和亚细胞系统，以及(2)更好地了解新陈代谢 男性生殖系统的。归根结底，智能网的发展 一种能够评估一种潜在生殖毒性的体外系统 化学物质被设想出来了。

The reproductive system is a target of many chemicals that have wide-spread domestic, agricultural, or industrial use. While the mechanism of toxicity is unknown in most cases, it is apparent that the reproductive system of the male is sensitive to chemicals such as dibromochloropropane, the alkyl glycol ethers, and certain phthalate esters. The study of the mechanisms of reproductive toxicants is hampered by the complexity of the physiological and biochemical processes of spermatogenesis. However, the functional unit of the testes is the seminiferous tubule, and it is relatively easy to isolate and culture its primary cell types. This simplified system will be used to examine the effects of the chemicals listed above under the carefully controlled conditions of tissue culture. In brief, the objectives are: (1) to determine the toxic mechanism of three groups of reproductive toxicants by the use of isolated testicular cell and subcellular systems, and (2) to better understand the metabolism of the male reproductive system. Ultimately, the development of an in vitro system capable of assessing the potential reproductive toxicity of a chemical is envisioned.