RETINAL NEUROTRANSMITTERS AND SYNAPTIC CIRCUITRY

猫视网膜中的神经元的化学特异性亚群将是 通过使用神经递质物质作为细胞化学物质 标记在光和电子显微镜研究。 鲜明 各亚群的形态特征和突触关系 将描述 放射自显影技术将用于研究 积累甘氨酸、GABA和牛磺酸的亚群。 视网膜 以神经活性肽为特征的神经元，包括P物质， 生长抑素和脑啡肽将通过使用两者来鉴定 放射自显影和免疫细胞化学技术。 电子致密 标记物，5，6-二羟色胺，将用于识别神经元， 使用多巴胺和吲哚胺作为递质。 功能途径将 通过神经递质的放射自显影分析进行研究， 2-在受控刺激条件下的脱氧葡萄糖摄取。 的影响 光与暗，视杆细胞与视锥细胞刺激，以及不同波长的光 将被调查。 化学结构之间的解剖学关系 将通过使用双标记来探索定义的亚群 其中放射自显影术将与高尔基体浸渍相结合的研究， 免疫细胞化学和逆行转运。 的识别 视网膜神经元的形态学上不同的亚群， 特定的化学递质和刺激条件的定义 影响这些发射器释放的因素将为 整合解剖学、神经化学和生理学数据。 阐明猫视网膜中的结构和化学回路将 提供有关视觉初步处理的基本信息 哺乳动物视网膜中的信息。

Chemically specific subpopulations of neurons in the cat retinal will be identified through the use of neurotransmitter substances as cytochemical markers in light and electron microscopic studies. Distinctive morphological features and synaptic relationships of each subpopulation will be described. Autoradiographic techniques will be used to study subpopulations which accumulate glycine, GABA, and taurine. Retinal neurons characterized by neuroactive peptides including substance P, somatostatin, and enkephalin will be identified by the use of both autoradiographic and immunocytochemical techniques. The electron dense marker, 5,6-dihydroxytryptamine, will be used to identify neurons which employ dopamine and indoleamine as transmitters. Functional pathways will be studied through autoradiographic analysis of neurotransmitter and 2-deoxyglucose uptake under controlled stimulus conditions. The effects of light vs. dark, rod vs. cone stimulation, and varying wavelengths of light will be investigated. The anatomical relationships between chemically defined subpopulations will be explored through the use of double labeling studies in which autoradiography will be combined with Golgi impregnation, immunocytochemistry and retrograde transport. The identification of morphologically distinct subpopulations of retinal neurons which utilize specific chemical transmitters and the definition of stimulus conditions which influence the release of these transmitters will provide a basis for integration of anatomical, neurochemical, and physiological data. Elucidation of structural and chemical circuitry in the cat retina will furnish basis information regarding the initial processing of visual information in a mammalian retina.