PROBLEMS IN EYE INFECTIONS

我们建议研究局部眼部因素在发病机制中的作用 和控制眼睛的单纯疱疹病毒（HSV）感染。 在 在这里提出的实验中，我们将：（1）测试角膜标本， 疱疹性眼病患者和对照病例，以揭示潜在的 角膜病毒感染;（2）建立角膜病毒感染的动物模型 潜伏期;（3）检查局部（局部）介质的作用，特别是 肾上腺素能药物对实验性眼部反复病毒脱落的影响 动物模型;（4）评价这些相同介质对HSV的作用 在角膜器官培养和细胞培养系统中复制。 以确定角膜中潜伏HSV的患病率和发病机制 通过共培养和免疫化学染色的角膜从 角膜移植术时患有疱疹性眼病的患者。 我们还将开发 动物角膜中HSV潜伏期的体外模型和比较再活化 感染的角膜和神经节 我们还将研究潜伏感染者的眼部病毒脱落， 局部应用肾上腺素能药物和其他介质后的动物 (e.g.干扰素）来检验局部 这些因素特别影响眼组织支持 HSV复制。 这些化合物将在潜伏感染的 动物、角膜器官培养物和细胞培养系统。 这个目标 研究是检查与特定介质的局部治疗， 预防或增强人类复发性疱疹性眼病的潜力。

We propose to study the effect of local ocular factors on the pathogenesis and control of herpes simplex virus (HSV) infections of the eye. In the experiments proposed here we will: (1) Test corneal specimens from patients with herpetic eye disease and from control cases to reveal latent virus infection of the cornea; (2) develop animal models of corneal latency; (3) examine the effect of topical (local) mediators, particularly adrenergic drugs, on recurrent viral shedding from the eye in experimental animal models; (4) evaluate the effect of these same mediators on HSV replication in corneal organ culture and cell culture systems. To prevalence and pathogenesis of latent HSV in cornea will be determined by co-cultivation and immunochemical staining of corneas removed from patients with herpetic eye disease at keratoplasty. We will also develop an in vitro model of HSV latency in animal corneas and compare reactivation in infected corneas and ganglia. We will also study the ocular shedding of virus in latently infected animals following locally applied adrenergic drugs and other mediators (e.g. prostaglandins, interferons) to test the hypothesis that local factors specifically affect the ability of the ocular tissues to support HSV replication. These compounds will be tested in latently infected animals, corneal organ cultures and cell culture systems. The goal of this research is to examine local treatment with specific mediators for their potential to prevent or enhance recurrent herpetic eye disease in humans.