VISUAL TRANSDUCTION IN PRIMATE PHOTORECEPTORS

我们建议研究人类视杆细胞和视锥细胞的视觉传导 短束猕猴，一种具有与人类相似的光感受器的猴子。 我们将记录单个细胞的光电流，以确定它们的 响应特性、电噪声和光谱灵敏度。这 实验信息将被用来开发一幅定量的图画 灵长类的光感受器编码视觉刺激的方式。 我们打算探讨以下问题： 1)棒和三种棒的光电流的动力学是什么？ 蛋筒的吗？ 2)强度信息是如何表示的？ 3)背景光有多大，亮度范围是多少？ 脱敏？ 4)黑暗中和暴露在空气中后，存在什么电噪声 明亮的(漂白)光？ 5)棒材和三种锥体的光谱灵敏度是多少 整个光谱的可见区域？ 6)转导机制的功能参数有变化吗？ 系统地在杆的外段中具有纵向位置 那圆锥体呢？ 7)灵长类的光感受器是电耦合的吗？

We propose to examine visual transduction in rods and cones of humans and Macaca fascicularis, a monkey with photoreceptors similar to those of man. We will record the photocurrents of single cells to determine their response properties, electrical noise, and spectral sensitivities. This experimental information will be used to develop a quantitative picture of the way in which primate photoreceptors encode visual stimuli. We intend to approach the following questions: 1) What are the kinetics of the photocurrents of rods and the three kinds of cone? 2) How is intensity information represented? 3) How much, and over what range of intensities do background lights desensitize? 4) What electrical noise is present in darkness and after exposure to bright (bleaching) light? 5) What is the spectral sensitivity of the rods and the three kinds of cone throughout the visible region of the spectrum? 6) Do the functional parameters of the transduction mechanism vary systematically with longitudinal position in the outer segments of the rods and cones? 7) Are primate photoreceptors electrically coupled?