MICROENVIRONMENT OF THE MAMMALIAN RETINA

拟议的工作涉及哺乳动物视网膜的氧合， 视神经头和光感受器的代谢， 氧利用率和对细胞外钾的影响。 的目的 该项目的各个组成部分是1）调查过程 负责光感受器的高耗氧量，2） 开发一个数学模型，将氧气测量与代谢 变化，3）研究灵长类动物中央凹的氧合，4）研究 视神经头的氧合在正常和 升高的眼内压，和5）研究碳的影响 对视网膜氧合的影响 视网膜供氧的变化与许多视网膜病变有关， 疾病，了解正常的视网膜氧合可以提供 深入了解这些疾病的机制和治疗方法。 特别是， 测量灵长类动物的中央凹氧分布， 关于代谢差异是否会导致 人的中央凹比其他视网膜更容易发生退行性变化， 地区 此外，视神经昏迷损伤的机制是 有争议的，这里使用的技术可以补充其他类型的 研究这个重要问题。 微电极将用于记录氧分压，钾 浓度和电活动的完整的眼睛麻醉 猫和猴子 要研究的自变量包括 光适应、血气和眼内压。 数据将 进行数学分析，以增强我们对氧气的理解 利用率

The proposed work concerns the oxygenation of the mammalian retina and optic nerve head, and the metabolism of photoreceptors with regard to their oxygen utilization and effect on extracellular potassium. The aims of individual components of the project are 1) to investigate the processes responsible for the high oxygen consumption of the photoreceptors, 2) to develop a mathematical model linking oxygen measurements with metabolic changes, 3) to study the oxygenation of the fovea in a primate, 4) to study the oxygenation of the optic nerve head under conditions of normal and elevated intraocular pressures, and 5) to study the effects of carbon dioxide on retinal oxygenation. Changes in the oxygen supply to the retina are implicated in many retinal diseases, and an understanding of normal retinal oxygenation may provide insight into mechanisms and therapies for these diseases. In particular, measurements of foveal oxygen distribution in the primate may yield information relevant to the question of whether metabolic differences make the human fovea more susceptible to degenerative changes than other retinal regions. Also, the mechanism of glaucomatous damage to the optic nerve is controversial, and the techniques used here can complement other types of studies on this important question. Microelectrodes will be used to record oxygen tension, potassium concentration and electrical activity of the intact eye of anesthetized cats and monkeys. Independent variables to be studied include the state of light adaptation, blood gases and intraocular pressure. Data will be subjected to mathematical analysis to enhance our understanding of oxygen utilization.