FRACTIONATION AND CHARACTERIZATION OF HUMAN OCULAR MUCUS

拟议研究的目标是提供信息，以便 对人类泪膜的稳定性有一个清晰的了解 患病的眼睛，基于各种泪液成分所扮演的角色。我们 建议隔离和/或确定负责组件。特价 将注意粘蛋白的分离。其他重要的撕裂 要研究的成分还包括血浆蛋白质，脂质和 酵素。将应用或开发微技术。方法： 分离和纯化将包括选择性溶剂提取、凝胶 离子交换层析、区带电泳法、梯度 超速离心法、免疫学方法等。土壤中的微量分析 单个组件将为表征和 身份证明。分离株的化学分析将包括碳水化合物 氨基酸分析仪上的GLC、氨基酸和一些氨基糖，以及 薄层扫描法测定脂类成分。几种重要酶的比活力 将会被化验。各个组件的物理属性将包括 粘度、溶解度、相对分子质量、表面活性和模式 结构元素的约束。我们对这一课题的初步研究： A)大分子组分的分级和部分表征 来自人眼粘液；b)糖蛋白中的O-糖苷键 从人的眼部粘液中分离出来的，表明我们将经受住极好的 实现我们目标的机会。

The objective of the proposed research is to yield information which will develop a clear understanding of human tear film stability in normal and diseased eyes, based on the roles taken on by various tear components. We propose to isolate and/or identify the responsible components. Special attention will be paid to the isolation of mucin. Other important tear components to be studied will also include plasma proteins, lipids, and enzymes. Microtechniques will be applied or developed. Methods of isolation and purification will include selective solvent extraction, gel and ion exchange chromatography, zone electrophoresis, gradient ultracentrifugation, immunological methods, and others. Microanalyses of individual components will provide a basis for the characterization and identification. Chemical analyses of isolates will include carbohydrates on GLC, amino acids and some amino sugars on the amino acid analyzer, and lipid components by TLC. Specific activities of some enzymes of importance will be assayed. Physical properties of individual components will include viscosity, solubility, molecular weight, surface activities and mode of binding of structural elements. Our preliminary studies on the subject: a) Fractionation and partial characterization of macromolecular components from human ocular mucus; b) O-Glycosidic linkage in the glycoprotein isolates from human ocular mucus, indicate that we will stand an excellent chance to achieve our objectives.