OUTER PLEXIFORM LAYER STRUCTURE AND FUNCTION

建议继续研究视网膜的结构和功能， 蛙的神经元。 水平细胞和受体端树突的研究 将在最初的资助期内完成。 这一更新提案 涉及双极细胞和无长突细胞。 程序包括细胞内记录，以建立基本的接受 场特性和HRP注射和形态测量研究， 注射细胞的电子显微镜检查。 这些数据将与 从高尔基体制备物和连续光和电子 标准制备物的显微镜检查。 对于双极，该研究将细胞功能与树突细 结构，识别的受体接触，一般形态， 分支和接触的精细结构和分布 端枝。 对于无长突细胞，细胞功能将与 工艺模式和程度，精细结构和分布的接触。 含有儿茶酚胺和吲哚胺的内层视网膜细胞将被破坏。 通过使用甲醛诱导的光学和电子显微镜表征 组织荧光和选择性变性程序。 的形态 这些细胞将与高尔基体和HRP的结果相关联 问题研究

It is proposed to continue a study of the structure and function of retinal neurones of Rana. Studies of horizontal cells and receptor telodendria will be completed during the initial grant period. This renewal proposal concerns bipolar and amacrine cells. Procedures include intracellular recording to establish basic receptive field properties and HRP injectioned and morphometric studies by light and electron microscopy of injected cells. These data will be correlated with quantitative data from Golgi preparations and serial light and electron microscopy of standard preparations. For bipolars, the study will correlate cell function with dendritic fine structure, identified receptor contacts, general morphology, level of branching and the fine structure and distribution of contacts on telodendria. For amacrine cells, cell function will be correlated with process pattern and extent, fine structure and distribution of contacts. Catecholamine and Indoleamine containing cells of the inner retina will be characterized by light and electron microscopy using formaldehyde induced histofluorescence and selective degeneration procedures. The morphology of these cells will be correlated with the results of the Golgi and HRP studies.