AQUEOUS HUMOR DYNAMICS AND VANADATE

钒，一种钠-钾ATPase抑制剂和腺苷环化酶刺激剂， 发现当局部应用于眼部时，可以降低眼压 兔子和猴子。因为这类化合物在治疗中可能有用 对于青光眼，本提案将研究钒酸盐的疗效、毒性和 猴子眼中的行为方式。单次和多次剂量反应 研究将进行测量眼压、色调流出量 设施，房水流量，荧光分光光度和巩膜外静脉压 被麻醉的猴子。钒酸盐对房水和/或睫状体的影响 测定环磷酸腺苷、腺苷环化酶和(Na+K)-ATPase水平 并与其对房水动力学的影响相关。眼内 钒酸盐激光治疗猴眼青光眼的压力效应 将检查流出通道的燃烧情况。

Vanadate, a sodium-potassium ATPase inhibitor and adenylate cyclase stimulator, was found to lower intraocular pressure when topically applied to the eyes of rabbits and monkeys. As this class of compound may be useful in the treatment of glaucoma, the present proposal will study vanadate's efficacy, toxicity and mode of action in the eyes of monkeys. Single and multiple dose-response studies will be carried out measuring intraocular pressure, tonographic outflow facility, aqueous flow by fluorophotometry and episcleral venous pressure in the anesthetized monkey. The effect of vanadate on aqueous and/or ciliary body levels of cyclic AMP, adenylate cyclase and (Na plus K)-ATPase will be measured and correlated with its effect on aqueous humor dynamics. The intraocular pressure effect of vanadate treatment of monkey eyes made glaucomatous by laser burning of the outflow pathways will be examined.