EFFECTORS AND REGULATORS OF DECAPENTAPLEGIC ACTIVITY

去瘫活动的效应器和调节器

• 批准号: 2392319
• 负责人: STEVEN D PODOS
• 金额: $ 2.01万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2392319
• 关键词: Drosophilidae; biological signal transduction; gene induction /repression; genetic regulatory element; transforming growth factors

The decapentaplegic (dpp) gene in Drosophila encodes a secreted protein of the transforming growth factor beta superfamily of proteins, which, like its counterparts in vertebrates, participates in numerous developmental processes from embryogenesis into adulthood. In the embryo, dpp exhibits the properties of an extracellular morphogen, conveying spatial information along the dorsal-ventral axis of the embryo. The zerknullt (zen) gene is activated by dpp in a dorsal stripe, and is required for formation of the amnioserosa. This proposal outlines three experiments to address the question of how the dpp signal is transduced from outside the cell to the nucleus. The first two lines of experiments seek to use the power of Drosophila genetics to identify genes required for proper zen activity, looking for dominant enhancers and suppressors or weak zen mutants. Such mutations could define genes that act in the dpp signal transduction pathway. Experiments are presented to distinguish where within the pathway each gene acts. The third line of experiments will complement the genetic analysis of the system and will involve a molecular dissection of the promoter region of the zen gene. Together these experiments should shed light on how signals are transduced downstream of TGF-beta family members and could provide insight into the roles of TGF-beta during mammalian development.

果蝇的dpp基因编码一种分泌蛋白 转化生长因子β超家族的蛋白质， 像脊椎动物中的对应物一样， 从胚胎发育到成年的发育过程。 在 在胚胎中，DPP表现出细胞外形态原的性质， 沿着背腹轴传递空间信息 胚胎 zerknullt（zen）基因在背纹中被dpp激活， 并且是浆膜层形成所必需的。 该提案概述了 三个实验来解决民进党信号如何 从细胞外传导到细胞核。 的前两行 实验试图利用果蝇遗传学的力量来识别 基因所需的适当zen活动，寻找显性增强子 和抑制者或弱禅突变体。 这样的突变可以定义基因 在DPP信号转导通路中起作用。 实验 以区分每个基因在通路中的作用。 的 第三行实验将补充基因分析， 系统，并将涉及启动子区的分子解剖， 禅宗基因 这些实验应该能揭示 信号在TGF-β家族成员的下游转导， 提供深入了解TGF-β在哺乳动物发育过程中的作用。