DETERMINANTS OF OPTIC AXON OUTGROWTH

一种针对细胞表面蛋白的抗体被鉴定出来，命名为M6， 在体外阻止神经突起的生长。关于显影光学的研究 神经在体内表明，M6只有在一定的临界水平上才能表达 发展时期。体外生物学功能及其模式的研究 体内的表达表明，通过更多地了解动物的行为 抗原及其抗体，我们将进一步了解为什么光学 轴突在发育过程中停止生长以及为什么它们通常不能 受伤后会再生。过去一年收集的其他结果 确认这一预期，并表示对 以初级视神经通路为中心的抗原及其抗体是 这是正当的。 这项建议集中在三个方面：(一)我们希望研究 假设抗原在体内参与了 轴突生长和细胞迁移。(2)我们希望使用该抗体来 不可逆转地阻止细胞迁移和轴突生长作为一种工具 研究开发过程中和开发后发生的各种相互依赖关系 受伤。(3)我们希望研究神经元是如何发展的 可以调节M6抗原的表达，从而修改 轴突生长和细胞迁移模式。 提出了九个单独的实验，每个实验都解决了一个或 这三个方面提出了更多的建议。总的来说，他们应该提供洞察力 一种似乎调节轴突的抗原在体内的行为 一种正在平行研究的分子机制的延伸 学习。他们还将利用抗体作为探针 以及用于研究视觉的可塑性和再生的标签 系统。

An antibody has been identified to a cell surface protein, designated M6, which blocks neurite outgrowth in vitro. Studies on the developing optic nerve in vivo indicate that M6 is expressed only up to a certain critical period of development. The biological function in vitro and the pattern of expression in vivo suggested that by knowing more about the behavior of the antigen and its antibody, we would gain further insight into why optic axons stop growing during development and why they generally fail to regenerate after injury. Additional results collected over the past year confirm this expectation and indicate that a substantial investigation of the antigen and its antibody centered on the primary optic pathway is justified. This proposal focuses on three areas: (1) We wish to examine the hypothesis that the antigen is involved in vivo with the processes of axonal growth and cell migration. (2) We wish to use the antibody to arrest irreversibly both cell migration and axonal outgrowth as a tool for studying various interdependencies occurring during development and after injury. (3) We wish to examine how the context in which a neuron develops may regulate the expression of the M6 antigen and as a result modify neurite outgrowth and cell migration patterns. Nine individual experiments are proposed, each of which addresses one or more of the three areas raised. Collectively, they should provide insight into the behavior in vivo of an antigen which appears to regulate neurite extension by a molecular mechanism which is under investigation in parallel studies. They will also exploit the use of the antibody both as a probe and a label for investigating plasticity and regeneration of the visual system.