CHEMICAL STUDIES OF ENZYMES AND PROTEINS

该项目包括对三种不同的生化系统的研究 意义：乳糖合成酶、人血清转铁蛋白及其细胞 受体和蛋白质的酶促N-糖基化。中的一个相连的主题 这项调查是对分子位置的鉴定 α-乳清蛋白和半乳糖基转移酶上的相互作用部位 乳糖合成酶及其受体相互作用部位的蛋白质组分 转铁蛋白。方法将包括差动标记法，共价 双官能团试剂的交联化及碎片的检测 对相互作用的抑制作用。结构与结构的关系 此外，还将研究单个蛋白质的功能。 蛋白质N-糖基化的研究将集中在多肽类似物的使用上 含有修饰的糖基化信号序列作为抑制剂和活性部位 共价标记。

The project encompasses studies of three systems of distinct biochemical significance: lactose synthase, human serum transferrin and its cellular receptors and the enzymatic N-glycosylation of proteins. A connecting theme in the investigation is the identification of the molecular locations of the interaction sites on alpha-lactalbumin and galactosyltransferase, the two protein components of lactose synthase, and of the receptor interaction site of transferrin. Approaches will include differential kinetic labelling, covalent crosslinking with bifunctional reagents as well as the examination of fragments for inhibitory effects on the interactions. The relationship of structure and function in the individual proteins will also be investigated. Studies of protein N-glycosylation will focus on the use of peptide analogues containing modified glycosylation signal sequence as inhibitors and active site covalent labels.