The UK Crop Microbiome CryoBank

该项目，英国作物微生物组低温库（UKCMCB）将建立一个与英国主要作物微生物组相关的微生物和信息的总资源。它汇集了四个领先的机构：Rothamsted Research，CABI，James赫顿研究所和John Innes中心（与UEA合作）。每个人都有与工业界合作的良好记录，在纯农业和应用农业研究的最前沿工作。植物微生物组主要由与植物相关的真菌，细菌和病毒组成，包括可以分离和培养的微生物和目前不适合培养的微生物。微生物聚生体包括通过提供营养来帮助植物宿主、帮助预防疾病或允许植物耐受环境条件的成员。在学术研究和农业技术行业需求的推动下，它将为作物微生物组研究提供资源，提供可持续的解决方案，以改善植物健康和作物生产力。该资源将有助于更好地了解微生物群落的相互作用，包括宿主植物和“植物生物群系”的其他组成部分，从而影响植物健康，从改善根际健康到控制生物威胁。该资源将包括：-一个公开的综合Cryobank收集的样品（根面材料-土壤，细菌和真菌分离物，植物材料和DNA），最初从315个样品中提取，这些样品来自对英国农业技术部门具有重要意义的系统。最初的重点将是来自英国9个不同土壤地点的6种作物（大麦、燕麦、油菜、马铃薯、甜菜和小麦）。这将用来自样品的可培养材料补充。样品将使用最先进的技术在超低温下进行最佳保存。一个精心策划的AgMicrobiome样本信息库，为最终用户提供注释序列和元数据。这将是第一个涵盖相同土壤类型中各种作物总微生物组的同步资源，由生物信息学资源，微生物学家，植物和作物健康专家以及世界一流的存储设施提供支持。提供材料将有助于对未开发的文化生物多样性进行研究。- 进一步的工作包将集中在展示UK-CMCB用于分离植物生长促进细菌和合成群落构建的实用性。这将涉及与英国作物相关的可培养微生物群的特征，以及与作物相关的合成微生物群落（SynComs）的生成和阳性植物生长性状的测试。通过这个工作包产生的微生物将被添加到CryoBank中，并向公众提供。植物培养和微生物分离工作将在Rothamsted Research进行，生物资源将与CABI的国家收藏馆一起保存和管理，而JHI & Rothamsted将管理序列资源的功能数据的生成。JIC将与UEA合作开展综合社区建设工作。样本将进行微生物群落分析，所有微生物分离株将进行系统发育表征，其中一个子集将进行全基因组测序。所有Meta基因组和基因组将在序列注释后保存在一个免费访问的数据库资源中，并为研究界提供微生物基因组资源，这将导致创建一个独特的，世界领先的微生物组材料，微生物，DNA和相关数据的组合资源，可用于学术和商业研究，并有可能用于可持续农业

This project, The UK Crop Microbiome Cryobank (UKCMCB) will establish a total resource of microorganisms and information associated with the microbiome of the UKs major crops. It brings together four leading institutions: Rothamsted Research, CABI, the James Hutton Institute and the John Innes Centre (in association with UEA). Each has a proven track record of working with industry, working at the forefront of pure and applied Agritech research.The plant microbiome mainly consists of fungi, bacteria and viruses that are associated with a plant and includes microbes that can be isolated and cultured and those that currently are currently not amenable to culture. Microbial consortia include members that help the plant host by providing nutrients, help prevent disease or allow a plant to tolerate environmental conditions. Driven by academic research and Agritech industry needs, it will provide a resource to underpin research on the Crop Microbiome, delivering sustainable solutions to improve plant health and crop productivity. The resource will facilitate better understanding of microbial community interactions, including the host plant and other components of the 'Phytobiome', and thereby impact plant health, from improvements in rhizosphere health through to control of biological threats. The resource will comprise:-A publicly available integrated Cryobank collection of samples (rhizoplane material -soils, bacterial and fungal isolates, plant material, and DNA) taken initially from 315 samples from systems significant to the UK Agritech sector. Initial focus will be on 6 crops (barley, oats, oil seed rape, potato, sugar beet and wheat ) from 9 different soil sites from across the UK. This will be supplemented with culturable material from the samples. Samples will be optimally preserved at ultra-low temperature using state-of-the-art technologies.-A curated AgMicrobiome Base of sample information with annotated sequences and meta-data for end-users. This will be the first synchronised resource covering the total microbiome of a variety of crops in identical soil types, supported by a bioinformatics resource, microbiologists, plant and crop health experts, with world class storage facilities. Provision of material will allow research into unexplored cultural biodiversity. - A further work package will be focussed on demonstrating the utility of the UK-CMCB for isolation of plant growth promoting bacteria and synthetic community construction. This will involve characterisation of the culturable microbiota associated with UK crop plants and the generation of crop-associated synthetic microbial communities (SynComs) and testing for positive plant growth traits. The microbes generated through this work package will be added to the CryoBank and made available to the public. The plant culture and microbial isolation work will take place at Rothamsted Research, biological resources will be held and curated in association with national collections at CABI, while JHI & Rothamsted will manage generation of functional data for the sequence resource. JIC in association with UEA will undertake the work on synthetic community construction. Samples will undergo microbial community profiling, and all microbial isolates will undergo phylogenetic characterisation and a subset of these will undergo full genome sequencing. All meta genomes and genomes will be deposited in a freely accessible database resource after sequence annotation, and provide a microbial genome resource for the research communityThis will result in the creation of a unique, world-leading combined resource of microbiome material, microorganisms, DNA and associated data useful for both academic and commercial research with potential for deployment in sustainable Agriculture