Beneficial Effects of Ascophyllum nodosum extract on crop microbiome, soil fertility and crop growth

瘤叶藓是一种丰富的棕色大型藻类，分布在沿着北部的潮间带 北美洲和欧洲的大西洋海岸线。许多研究报告探讨了 A.节瘤草提取物对植物生长发育的影响许多潜在的潜在生理机制 在提高生产力、植物活力、植物营养和作物肥力方面， 处理A.结节提取物（ANE）。宏量和微量元素营养素、氨基 酸、维生素和植物生长刺激物质（例如细胞分裂素，生长素， 和赤霉酸样物质）与海藻提取物的生长促进作用有关 对植物生长的影响。然而，很少有人知道海藻提取物之间的相互作用， 植物根系和有益的土壤微生物（如细菌和真菌）的非凡多样性， 根际这些土壤微生物通过各种方式有益于其宿主植物的生长，通常通过 促进养分的获取，例如：已知促进植物生长的丛枝菌根真菌 通过增加矿物质吸收，特别是磷的生长。 该项目的目的是与印度尼西亚海洋植物有限公司（ASL）建立合作关系， 更好地理解的潜在机制所产生的有益影响，在A。结节性 植物生长，特别是植物-微生物相互作用的提取物。本研究将集中于 菌根真菌以及根际和内生细菌和真菌。我们将使用温室试验， 针对细菌的16 S rRNA基因、真菌的ITS基因和菌根的18 S rRNA基因的扩增子测序 Illumina MiSeq Technology上的真菌。

Ascophyllum nodosum (L) Le Jol. is an abundant brown macro-alga found in intertidal zones along the North Atlantic coastlines of North America and Europe. Many research reports have explored the beneficial effects of A. nodosum extracts on plant growth and development. Many potential underlying physiological mechanisms have been indicated in terms of enhance productivity, plant vigor, plant nutrition, and crop fertility following treatments with A. nodosum extracts (ANE). Components such as macro- and micro-element nutrients, amino acids, vitamins, and the endogenous stimulation of plant growth stimulating substances (e.g. cytokinins, auxins, and gibberellic acid-like substances) have been implicated in the growth promotion effects of seaweed extracts on plant growth. However, little is known about the effects of seaweed extracts on the interactions between plant roots and the extraordinary diversity of beneficial soil microbes (e.g. bacteria and fungi) in the rhizosphere. These soil microbes benefit the growth of their host plants by various means, commonly by facilitating access to nutrients, as an exmaple: Arbuscular mycorrhizal fungi that are known to promote plant growth by enhancing mineral uptake particularly phosphorus. The aim of this project is to establish a collaboration with Acadian Seaplants Ltd (ASL) in order to develop a better understanding of the underlying mechanisms resulting from the beneficial effects of in A. nodosum extracts on plant growth, and specifically on plant-microbial interactions. This research will focus on mycorrhizal fungi as well as rhizospheric and endophytic bacteria and fungi. We will use greenhouse trials and amplicon sequencing targeting 16S rRNA gene for bacteria, ITS for fungi and 18S rRNA gene for mycorrhizal fungi on Illumina MiSeq Technology.