Beneficial Effects of Ascophyllum nodosum extract on crop microbiome, soil fertility and crop growth

结瘤隐孢霉Acophhyllum nodosum(L)Le Jol.是一种在北部潮间带发现的丰富的棕色大型藻类 北美和欧洲的大西洋海岸线。许多研究报告都探讨了以下几点： 节瘤参提取物对植物生长发育的影响。许多潜在的潜在生理机制 已经在提高生产力、植物活力、植物营养和作物肥力方面得到了表明 结瘤参浸液处理(ANE)。常量和微量营养素、氨基酸等成分 酸、维生素和植物生长刺激物质的内源刺激(如细胞分裂素、生长素、 和类似赤霉酸的物质)与海藻提取物的促生长作用有关。 对植物生长的影响。然而，海藻提取物对相互作用的影响却知之甚少。 植物根部和有益土壤微生物(如细菌和真菌)的非凡多样性 根际。这些土壤微生物通过各种方式有利于寄主植物的生长，通常是通过 促进营养的获取，例如：已知能促进植物生长的丛枝菌根真菌 通过增加矿物质的吸收，特别是磷的吸收来生长。 该项目的目的是与阿卡迪亚海洋植物有限公司(ASL)建立合作，以开发一种 更好地理解根瘤拟青霉有益作用的潜在机制 提取物对植物生长，特别是对植物与微生物的相互作用。这项研究将集中在 菌根真菌以及根际和内生细菌和真菌。我们将使用温室试验和 细菌16S rRNA基因、真菌ITS和菌根18S rRNA基因的扩增序列分析 Illumina MiSeq技术上的真菌。

Ascophyllum nodosum (L) Le Jol. is an abundant brown macro-alga found in intertidal zones along the North Atlantic coastlines of North America and Europe. Many research reports have explored the beneficial effects of A. nodosum extracts on plant growth and development. Many potential underlying physiological mechanisms have been indicated in terms of enhance productivity, plant vigor, plant nutrition, and crop fertility following treatments with A. nodosum extracts (ANE). Components such as macro- and micro-element nutrients, amino acids, vitamins, and the endogenous stimulation of plant growth stimulating substances (e.g. cytokinins, auxins, and gibberellic acid-like substances) have been implicated in the growth promotion effects of seaweed extracts on plant growth. However, little is known about the effects of seaweed extracts on the interactions between plant roots and the extraordinary diversity of beneficial soil microbes (e.g. bacteria and fungi) in the rhizosphere. These soil microbes benefit the growth of their host plants by various means, commonly by facilitating access to nutrients, as an exmaple: Arbuscular mycorrhizal fungi that are known to promote plant growth by enhancing mineral uptake particularly phosphorus. The aim of this project is to establish a collaboration with Acadian Seaplants Ltd (ASL) in order to develop a better understanding of the underlying mechanisms resulting from the beneficial effects of in A. nodosum extracts on plant growth, and specifically on plant-microbial interactions. This research will focus on mycorrhizal fungi as well as rhizospheric and endophytic bacteria and fungi. We will use greenhouse trials and amplicon sequencing targeting 16S rRNA gene for bacteria, ITS for fungi and 18S rRNA gene for mycorrhizal fungi on Illumina MiSeq Technology.