BIOLOGICAL ELECTRON TRANSFER

C型细胞色素电子传递机制的研究进展 已经产生了大量的信息，并使我们制定了一个数字 有趣的和潜在的重要问题，包括：角色 电子对蛋白质表面结构和蛋白质动力学的影响 调职。这项工作的最终目标仍然是理解 生物电子传递与能量守恒的耦合机制。 然而，为了充分理解这一进程，一些相关的问题必须 涉及生物电子转移和 保护结构和功能的一般领域。因此，一个不可分割的部分 阐明生物电子传递机制的重点是 两种蛋白质相互作用的化学和结构方面 这使这些反应具有高度的特异性和快速的反应速度。这个 这里提出的研究旨在解决这两个问题 生物电子传递机制及其性质 蛋白质之间的相互作用。具体来说，我们建议调查 细胞色素C与非血红素铁硫蛋白的相互作用 光合作用反应中心。我们还提议进行实验 旨在阐明细胞色素的表面结构和动力学。 解决方案，并利用这些信息来理解蛋白质-蛋白质 相互作用和电子转移。我们预计这些信息来自于 从这些研究中将不仅承载生物电子的机制 转让，但将提供有助于理解一般情况的信息 动力学在蛋白质功能和特定化学性质中的作用 和物理相互作用中的反应涉及相互作用 大分子。

Past studies on the mechanism of electron transfer by c-type cytochromes have yielded considerable information and have led us to formulate a number of interesting and potentially significant questions, including: the role of the protein surface structure and protein dynamics in electron transfer. The ultimate goal of this work remains to understand the mechanism of coupling biological electron transfer to energy conservation. However, to fully understand this process a number of related issues must be addressed which bear on both biological electron transfer and the general areas of prot ein structure and function. Thus, an integral part of elucidating the mechanism of biological electron transfer focuses on the chemical and structural aspects of the interaction between two proteins which give these reactions their high specificity and rapid rates. The studies proposed here are designed to address both the question of the mechanism of biological electron transport and the nature of protein-protein interactions. Specifically we propose to investigate the inter-actions of cytochrone c with non-heme iron sulfur proteins and photosynthetic reaction centers. We also are proposing experiments designed to elucidate the surface structure and dynamics of cytochromes in solution and to use this information in understanding protein-protein interactions and electron transfer. We anticipate that information derived from these studies will bear not only the mechanism of biological electron transfer but will provide information useful in understanding the general role of dynamics in protein function and the nature of specific chemical and physical inter-actions in reactions involving the interaction of macromolecules.