ACTIVE OXYGEN GENERATION BY HEPATIC MICROSOMES

我们认为细胞色素P-450混合功能氧化酶反应中含有 两个反应，一个是NADPH氧化酶，另一个是单加氧酶反应。至 研究单加氧酶反应的化学计量学的尝试一直是 对过氧化氢的产生感到困惑，过氧化氢是NADPH-氧化酶的一种成分。我们有 开发了一种包络这两种反应的机制，并将试图展示 在混合功能氧化酶反应中，活性氧解偶联 随后氧化的细胞色素P-450释放，产生过氧化氢。 考察了不同底物对化学计量比的影响。

We propose that the cytochrome P-450 mixed function oxidase reaction contains two reactions, one an NADPH oxidase and the other a monooxygenase reaction. To date attempts to study stoichiometries of the monooxygenase reaction have been confused by the generation of H2O2, a component of the NADPH-oxidase. We have developed a mechanism which envelopes both reactions and will attempt to show that during the mixed function oxidase reaction, uncoupling of active oxygen with subsequent discharge of oxygenated cytochrome P-450, yields H2O2. Influence of different substrates on the stoichiometries will be examined.