MUTATIONS AFFECTING THE PRODUCTION OF MATURE RNAS

在我们努力识别对前tRNA重要的基因产物时 剪接过程中，我们发现了意想不到的蛋白质类别 很可能在调节和亚细胞定位中发挥作用 剪接酶和前tRNA底物以及蛋白质 这很可能与前tRNA相互作用。我们对RNA1和RNA1的研究 SRN1将环境刺激(即葡萄糖)和RNA加工联系起来。我们的 RNA1和SFP1与核输入或核结构与RNA的关联研究 正在处理。我们对LOS1的研究表明，细胞核中的蛋白质 膜可以影响Pre-tRNA的加工，我们对Stp1p的研究提供了 辅助蛋白在酶促反应步骤中作用的证据 前tRNA剪接。拟议的工作旨在批判性地定义 这些基因产物中的每一个发挥作用的步骤和确定 它们相互作用的大分子，由五个特定的目标组成。 这些是为了：(A)确认RNA处理对葡萄糖和 研究这种环境信号的传递机制(S) 核糖核酸加工机械；(B)调查核胞质如何 沟通涉及RNA加工；(C)继续学习RNA1 以及它在协调环境刺激方面的核心作用， 核浆通讯和RNA加工；(D)确定 Los1p影响核内剪接内切酶的拓扑结构 以及(E)检验Stp1p影响前tRNA剪接的模型 内切酶相互作用。

In our efforts to identify gene products important to the pre-tRNA splicing process, we discovered unanticipated categories of proteins that are likely to play a role in the regulation and subcellular location of the splicing enzymes and the pre-tRNA substrates, as well as a protein that is likely to interact with the pre-tRNAs. Our studies of RNA1 and SRN1 link environmental stimuli (i.e., glucose) and RNA processing. Our studies of RNA1 and SFP1 link nuclear import or nuclear structure and RNA processing. Our studies of LOS1 indicate that proteins of the nuclear membrane can affect pre-tRNA processing and our studies of Stp1p provide evidence for the role of accessory proteins for the catalytic steps of pre-tRNA splicing. The proposed work is designed to critically define the steps at which each of these gene products functions and to determine the macromolecules with which they interact and consist of five specific aims. These are to: (A) confirm that RNA processing responds to glucose and study the mechanism(s) by which this environmental signal is transmitted to the RNA processing machinery; (B) investigate how nuclear-cytosol communication is involved with RNA processing; (C) continue studying RNA1 and its proposed central role in coordinating environmental stimuli, nuclear-cytosol communication and RNA processing; (D) determine whether Los1p affects the topology of splicing endonuclease in the nuclear membrane; and (E) test the model that Stp1p can affect pre-tRNA-splicing endonuclease interaction.