STRUCTURE-FUNCTION OF GLYCOSYLTRANSFERASES AND LECTINS

三组蛋白质作用的分子基础， 将研究涉及复杂碳水化合物功能的方面。 第一章 糖基转移酶类，催化低聚糖的合成， 将以几种方式检查糖缀合物。 A）选择的转移酶 将被分离并表征其性质。 B）抗 选择的转移酶将用于定位细胞内的酶， 检测它们是否以多酶复合物的形式存在， 转移酶的结构。 C）选定的生物合成作用 将寻求转移。 D）共价结合的可能作用 转移酶中的脂质以及生物脂质对 将检查所选转移酶的性质。 E）基因 选择的转移酶将被克隆以获得足够的酶， 结构/功能研究。 2)凝集素类。 脊椎动物肝凝集素， 首先，将从几个样品中检测岩藻糖特异性凝集素， 方面 A）针对凝集素的抗体将用于定义凝集素的特异性。 凝集素的亚细胞定位。 B）天然存在的配体， 将寻找凝集素。 C）岩藻糖凝集素的结构研究 将被执行。 D）凝集素在胚胎发育过程中的可能作用， 将寻求胎儿发育。 3)核苷酸糖用酶 相互转换。 在核苷酸糖生物合成中起作用的关键酶 将被孤立和特点，重点是了解 调节核苷酸糖生物合成的分子机制。

The molecular basis of the actions of three groups of proteins that are involved in aspects of complex carbohydrate function will be examined. 1) GLYCOSYLTRRANSFERASES that catalyze the synthesis of oligosaccharides in glycoconjugates will be examined in several ways. A) Selected transferases will be isolated and their properties characterized. B) Antibodies to selected transferases will be used to localize the enzymes intracellularly, to examine whether they exist as multienzyme complexes and as probes for the structure of the transferase. C) The biosynthetic roles of selected transferases will be sought. D) The possible role of covalently bound lipids in the transferases and the effect of biological lipids on the properties of selected transferases will be examined. E) Genes for selected transferases will be cloned to obtain sufficient enzyme for structure/function studies. 2) LECTINS. Vertebrate liver lectins, primarily the fucose-specific lectin will be examined from several aspects. A) Antibodies against the lectins will be used to define the subcellular localization of lectins. B) Naturally occurring ligands for the lectins will be sought. C) Structural studies on the fucose lectin will be performed. D) The possible roles of lectins during embryonic and fetal development will be sought. 3) ENZYMES FOR NUCLEOTIDESUGAR INTERCONVERSION. Key enzymes that act in nucleotide sugar biosynthesis will be isolated and characterized with emphasis on understanding the molecular mechanisms that act in regulation of nucleotidesugar biosynthesis.