Replacement fluorescence activated cell sorter for the Jenner Laboratory (BBSRC National Vaccinology Centre: The Jenner Building)

荧光激活细胞分选(FACS)是一种强大的技术，它能够测量单个细胞的特征，然后根据它们的特征分离这些细胞。数万个单独的细胞可以在几分钟内分离出来，然后进行分析。这对我们了解动物对病毒感染的反应至关重要，并使我们能够设计和开发病毒疫苗来预防疾病。皮尔布赖特研究所(Pirbright)的目的是预测、检测、了解和应对严重的家畜病毒性疾病和从动物传播到人类的病毒。皮尔布赖特在英国是独一无二的，为科学家提供了进入最先进的动物和实验室设施的机会，以便在高遏制水平下研究牲畜的传染病。位于皮尔布赖特的BBSRC国家疫苗中心(Jenner大楼)是一个低遏制设施，支持中等风险病毒的工作。该项目旨在确保Jenner大楼的FACS能力在未来10-12年内保持连续性。为了实现这一目标，我们建议用升级到BD FACSAria Fusion(Aria Fusion)的BD FACSAria U3(Aria U3)取代目前已有15年历史的BD FACSAria U3(Aria U3)。Aria Fusion具有卓越的多色性能、最新的软件和硬件以及增强的生物安全保护。微生物安全柜的增加将增加样品和操作员的保护。保留我们的FACS能力并扩大我们对病毒感染样本的分类能力将使我们能够更好地了解动物对病毒感染的反应。

Fluorescence-activated cell sorting (FACS) is a powerful technology that enables the measurement of characteristics of single cells and then separates these cells based on their characteristics. Tens of thousands of individual cells can be isolated within minutes and are then available for analysis. This is critical to our understanding of how animals respond to virus infections and enable us to design and develop viral vaccines to prevent disease.The Pirbright Institute's (Pirbright) purpose is to predict, detect, understand, and respond to serious viral diseases of livestock and viruses that spread from animals to humans. Pirbright is unique within the UK, providing scientists with access to both state-of-the-art animal and laboratory facilities to research infectious diseases of livestock at high containment levels. The BBSRC National Vaccinology Centre (Jenner Building) at Pirbright, is a low containment facility that supports work with medium risk viruses. This project aims to ensure continuity of the FACS capability in the Jenner Building for the next 10-12 years. To achieve this, we propose to replace our current 15 years old FACSorter, the BD FACSAria U3 (Aria U3), positioned within the Jenner Building, with an upgrade to a BD FACSAria Fusion (Aria Fusion).The Aria Fusion is superior to the Aria U3 with exceptional multicolour performance, up to date software and hardware and enhanced biosafety protection. The addition of the microbiological safety cabinet will increase sample and operator protection. Retaining our FACS capacity and expanding our ability to sort virus infected samples will enable us to understand better animal responses to virus infection.