STUDIES OF DNA POLYMERASES

培养人DNA聚合酶α、β和伽马的种类 成纤维细胞和HeLa细胞将被分离和研究关于 保真度、催化性能和功能。特别强调的是 影响成纤维细胞传代后期数量的变化 在生长过程中汇合。将进行抗体研究，以应对 阿尔法物种，以尝试设计亲缘关系柱 纯化，并理解 各种阿尔法亚种。人类聚合酶将在 各种底物，以确定它们在DNA修复中的作用 确定在这种能力下可能与聚合酶一起发挥作用的因素。E. ColiDNA聚合酶也将被研究。聚合酶I的一个新物种 在RecA诱导后出现的情况将被表征和活动 聚合酶II和III在各种受损底物上的 已经确定了。

Species of DNA polymerases Alpha, Beta, and Gamma from cultured human fibroblasts and HeLa cells will be fractionated and studied with regard to fidelity, catalytic properties, and function. Particular emphasis will be put upon changes that occur during late passage numbers of fibroblasts or during growth to confluence. Antibody studies will be carried out for the Alpha-species, in order to attempt to design affintiy columns for purification and to understand the significance and commonalities of the various Alpha-subspecies. The human polymerases will be tested upon various substrates in order to ascertain their role in DNA repair and to identify factors that may work with the polymerases in this capacity. E. coli DNA polymerases will also be studied. A new species of polymerase I that appears after recA induction will be characterized and the activities of polymerases II and III upon various damaged substrates will be ascertained.