CHROMOSOME STRUCTURE AND GENE ARRANGEMENT

这项研究的长期目标是深入了解 染色质结构和基因表达。中描述的具体目标 这一应用是：(1)获得有关分子的新信息 中期染色体中活性基因的构筑。中期 染色体和间期核将从同步化 鸡细胞。活性基因的组织，包括 甘油醛-3-磷酸脱氢酶、胸苷激酶和β-微管蛋白 染色体/核中的基因将使用核酸酶和 以这些基因为探针的重组DNA分子。这个 活性基因在中期染色体的纵向分布将是 使用染色体缺口平移法进行研究。(2)调查 早期的核骨架结构(联会复合体) 减数分裂细胞。抗联会复合体蛋白的单抗 将被制备并用作探针来研究结构和 核内联会复合体的生理作用。(3) 染色质结构在异常染色体中的同质性等 染色区域(HSR)和双分钟(Dm)，这是最常见的 在耐药突变和肿瘤细胞中观察到的，将被研究 同样使用重组DNA和核酸酶作为探针。其中的可能性 这种基因扩增可能发生在长春新碱耐药突变体中。 包含高铁和dm的将使用差值进行调查 大豆花叶病毒构建的cDNA文库或基因组DNA文库的筛选 变种人。从这些描述的项目中获得的结果可以 提供分子遗传学方面的有用信息，增强基础知识 人类疾病方面的知识。

The long-term objectives of this research are to gain insights into chromatin structure and gene expression. The specific aims described in this application are: (1) To gain new information concerning the molecular architecture of active genes in metaphase chromosomes. Metaphase chromosomes and interphase nuclei will be isolated from synchronized chicken cells. The organizations of active genes, including the glyceradehyde-3-phosphate dehydrogenase, thymidine kinase and Beta-tubulin genes, in chromosomes/nuclei will be investigated using nucleases and recombinant DNA molecules containing these genes as probes. The longtitudinal distribution of active genes in metaphase chromosomes will be studied using a chromosome nick-translation method. (2) To investigate the nuclear skeletal structure (synaptonemal complex) in prophase stage of meiotic cells. Monoclonal antibodies against synaptonemal complex proteins will be prepared and used as probes to investigate the structural and physiological roles of the synaptonemal complex inside the nuclei. (3) The chromatin structure in the abnormal chromosomes such as homogeneously staining regions (HSR) and double minutes (dm), which are most often observed in drug-resistant mutants and neoplastic cells, will be studied similarly using recombinant DNA and nucleases as probes. The possibilities that gene amplification may take place in the vincristine-resistant mutants which contain HSR and dm will be investigated using a differential screening method of a cDNA library or genomic DNA library constructed from the mutants. The results obtained from these described projects may provide useful information in molecular genetics and enhance fundamental knowledge in human diseases.