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STRUCTURE AND FUNCTION OF THE NUCLEAR ENVELOPE

STRUCTURE AND FUNCTION OF THE NUCLEAR ENVELOPE
核膜的结构和功能
批准号：
3281936
负责人：
JOHN W SEDAT
金额：
$9.28万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
1983
资助国家：
美国
项目状态：
已结题
起止时间：
1983-12-01 至 1986-11-30
来源：
https://reporter.nih.gov/project-details/3281936
关键词：
affinity chromatography chemical structure function early embryonic stage electron microscopy gel electrophoresis immunological substance monoclonal antibody nonmammalian vertebrate embryology nuclear membrane oogenesis surface antigens

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中文摘要
翻译
这一建议建立在已建立的针对 果蝇核蛋白及其最新开发工具 亚细胞结构的三维重建。 这一建议集中在标记核的抗体的子集上。 信封。使用电子显微镜，亚结构和位置在 核包层将被建立起来。进一步的相互关系 核包膜亚结构与间期染色体 将完成三维图像处理技术。 将对该抗原进行纯化，并对其进行广泛的生化鉴定 程序。
英文摘要
This proposal builds on the established library of monoclonal antibodies to Drosophila nuclear protein and the recently developed tools of three-dimensional reconstruction of subcellular structures. This proposal focusses on a subset of the antibodies that label the nuclear envelope. Using electron microscopy, the substructure and location within the nuclear envelope will be established. Further correlation of the nuclear envelope substructures to the interphase chromosomes by the three-dimensional image processing techniques will be accomplished. The antigen will be purified and extensively characterized by biochemical procedures.
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