ANTIBODY MODEL FOR RECOGNITION OF DNA HELICAL DIVERSITY

动物将用具有代表性的A、B和Z-DNA样本进行免疫 和定义序列的碱基配对寡核苷酸。由此产生的多克隆 单抗将被测试其区分能力。 其中：不同家族(A、B和Z)的螺旋；各种螺旋 一个家庭的结构；以及个体的各种地形特征 螺旋形。进一步的实验将测试抗体的能力。 检测插入的唯一依赖于序列的螺旋构象 线形或环状DNA分子及其对动态平衡的影响 在不同的螺旋构象之间，可以假设 序列。抗体也将被用来探测相应的唯一 自然产生的DNA、染色质和细胞核中的螺旋区。这些 实验将提供检验假说的试剂和程序。 特定的局部构象特征出现在染色质内 DNA的组织，在调控过程中可能作为信号位点 基因信息的转录或复制。

Animals will be immunized with representative examples of A, B and Z-DNA and base-paired oligonucleotides of defined sequence. Resulting polyclonal and monoclonal antibodies will be tested for their ability to distinguish among: helices of different families (A, B and Z); various helical structures of one family; and various topographical features of individual helical forms. Further experiments will test the ability of the antibodies to detect unique sequence-dependent helical conformation inserted into linear or circular DNA molecules and to affect the dynamic equilibrium between different helical conformations that can be assumed by a given sequence. Antibodies will also be used to probe for corresponding unique helical regions in naturally occurring DNA, chromatin and nuclei. These experiments will provide reagents and procedures for testing the hypothesis that specific local conformational features occur within the chromatin organization of DNA and may serve as signal sites in processes regulating transcription or replication of gene information.